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首页> 外文期刊>International Journal of Electrochemical Science >Fabrication of Inorganic Monolith Coated with Gold Nanoparticles for Protein Purification
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Fabrication of Inorganic Monolith Coated with Gold Nanoparticles for Protein Purification

机译：纳米金包覆的纳米蛋白质的整体制备工艺。

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This work aims at developing a new technique used to fabricate porous inorganic monolith using poresurface improved coverage with gold nanoparticles (AuNPs) that has the capacity of preconcentratingproteins that has effectiveness of high preconcentration. The advantage of incorporating inorganicmonolith with gold nanoparticles is getting high effective isolation of target analytes as a result of helpfulfeatures of surface area. Initially, the inorganic monolith was fabricated using the sol-gel process. Thefabricated monolithic material was then chemically modified using 3-mercaptopropyltrimethoxysilane,and subsequently AuNPs immobilization on the surface of the inorganic monolith was performed. Thematerials that were synthesized were studied with the use of diverse methods such as FT-IR analysis,UV-Vis spectroscopy, EDAX and TEM analysis. As a fabricated sorbent for preconcentration ofstandard proteins (hemoglobin and pepsin), the AuNPs modified inorganic monolith was applied and itsperformance was contrasted with bare inorganic monolith. The fabricated AuNPs modified inorganicmonolith would be a powerful sorbent for protein preconcentration from a real sample that iscomplicated.
机译：这项工作旨在开发一种新技术，该技术用于利用多孔表面改进的金纳米颗粒（AuNPs）覆盖率来制备多孔无机整体材料，该纳米颗粒具有预浓缩蛋白质的能力，并具有高预浓缩的功效。将无机整体材料与金纳米颗粒结合的优势是表面积的有用功能，可实现目标分析物的高效分离。最初，无机溶胶是使用溶胶-凝胶法制备的。然后，使用3-巯基丙基三甲氧基硅烷对所制备的整体材料进行化学改性，然后将AuNPs固定在无机整体材料的表面上。使用多种方法对合成的材料进行了研究，例如FT-IR分析，UV-Vis光谱，EDAX和TEM分析。作为预先浓缩标准蛋白（血红蛋白和胃蛋白酶）的吸附剂，使用了AuNPs改性的无机整料，其性能与裸露的无机整料形成对比。所制备的AuNPs修饰的无机整料将是从复杂的真实样品中进行蛋白质预浓缩的强大吸附剂。

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