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首页> 外文期刊>International Journal of Electrochemical Science >The Determination of Human Albumin by a Quartz Crystal Microbalance Immunosensor
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The Determination of Human Albumin by a Quartz Crystal Microbalance Immunosensor

机译：石英晶体微天平免疫传感器测定人白蛋白

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摘要

Albumin is a protein serving as a standard biochemical marker which is assayed in the blood and bloodplasma as a liver function test but its diagnostics importance is much wider. In the current time,albumin can be determined by standard immunochemical and spectral methods. In this work,immunosensor based on the piezoelectric10 MHz QCM was developed to determine albumin in levelscorresponding with expected concentration in blood plasma. The immunosensor contained an antibodyspecific to albumin interdigitated to electrode surface through protein A. The immunosensor wasperformed for the determination of albumin and limit of detection 0.234 mg/ml was achieved. Thelimit of detection is lower than the expected plasma level of albumin (35 – 55 mg/ml). Theimmunosensor assay fully correlated with the standard ELISA and the immunosensor exerted goodlong-term stability for at least two months. The immunosensor based determination of albumin appearsto be a promising tool for practical use in field or home care conditions. No elaborative manipulationwith samples or necessity to use specific reagents is a significant advantage of the immunosensorbased assay.
机译：白蛋白是一种用作标准生化标志物的蛋白质，可在血液和血浆中作为肝功能测试进行检测，但其诊断重要性却更为广泛。目前，白蛋白可以通过标准的免疫化学和光谱方法测定。在这项工作中，开发了一种基于压电10 MHz QCM的免疫传感器，以测定与血浆中预期浓度相对应的白蛋白水平。该免疫传感器包含特异于蛋白的抗体，该蛋白通过蛋白A与电极表面交错。该免疫传感器用于测定白蛋白，检出限为0.234 mg / ml。检测限低于预期的白蛋白血浆水平（35 – 55 mg / ml）。免疫传感器测定与标准ELISA完全相关，并且免疫传感器具有良好的至少两个月的长期稳定性。基于免疫传感器的白蛋白测定看来是在现场或家庭护理条件中实际使用的有前途的工具。基于样品的详尽操作无需使用样品进行详尽的操作，也无需使用特定试剂。

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