Glucose Biosensing at Carbon Paste Electrodes Containing Polyaniline-Silicon dioxide Composite

Halit Arslan; Merve ?zdemir; Huseyin Zengin; Gülay Zengin

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Glucose Biosensing at Carbon Paste Electrodes Containing Polyaniline-Silicon dioxide Composite

机译：含聚苯胺-二氧化硅复合材料的碳糊电极对葡萄糖的生物传感

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In this study, a novel a carbon paste electrode using the salt form of polyaniline (pani)-silicon dioxidecomposite sensitive to glucose, was prepared. Glucose oxidase enzyme was immobilized to carbonpaste electrode by cross-linking with glutaraldehyde. Determination of glucose was carried out by theoxidation of enzymatically produced H2O2 at 0.4 V vs. Ag/AgCl. The effects of pH and temperaturewere investigated and optimum parameters were found to be 9.0 and 60 C, respectively. The linear-6 -3 2working range of the electrode was 5.010 - 1.010 M, R =0.997. The storage stability andoperation stability of the enzyme electrode were also studied. The results showed that 98.4 % of theresponse current was retained after 16 activity assays. The prepared glucose biosensor retained 37 % ofinitial activity after 70 days when stored in 0.1 M phosphate buffer solution at 4 C.

机译：在这项研究中，制备了一种新型的碳糊电极，该电极使用了对葡萄糖敏感的聚苯胺（pani）-二氧化硅复合材料的盐形式。通过与戊二醛交联将葡萄糖氧化酶固定在碳糊电极上。葡萄糖的测定是通过在0.4 V相对于Ag / AgCl的酶促氧化过氧化氢中进行的。研究了pH和温度的影响，发现最佳参数分别为9.0和60℃。电极的线性-6 -3 2工作范围为5.010-1.010M，R ＝ 0.997。还研究了酶电极的储存稳定性和操作稳定性。结果表明，经过16次活性测定，保留了98.4％的响应电流。当在4 C下储存在0.1 M磷酸盐缓冲溶液中后，制备的葡萄糖生物传感器在70天后保留了37％的初始活性。

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《International Journal of Electrochemical Science》 |2012年第10期|共10页
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Halit Arslan; Merve ?zdemir; Huseyin Zengin; Gülay Zengin;
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Glucose Biosensing at Carbon Paste Electrodes Containing Polyaniline-Silicon dioxide Composite

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