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首页> 外文期刊>International Journal of Environment and Bioenergy >Enhancement of Hydrogen Production Using Microbial Electrolysis Cell
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Enhancement of Hydrogen Production Using Microbial Electrolysis Cell

机译：利用微生物电解池提高产氢量

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Fermentative hydrogen production was carried out in a sealed conical flask with provision for gas collection by water displacement method using facultative bacterial strain isolated from soil. Fermentation was carried out in liquid medium with maize stalk hydrolysate as carbon source. A maximum hydrogen yield of 62.3 mL H_2/L was obtained at the end of fermentation. The HPLC analysis of the fermentation effluent revealed the presence of fermentation end products like oxalic, tartaric, formic, pyruvic and citric acid. In the second stage, the fermentation effluent with the organic acids is used as a feed for a microbial electrolysis cell (MEC) for further hydrogen production. The cells were separated from the effluent by centrifuging. Anode (Type A carbon cloth) and cathode (Type B carbon cloth) of the MEC were kept 2 cm apart. The anode of the MEC was enriched in a microbial fuel cell with waste water inoculum. After passing nitrogen for 10 min, a voltage ranging from 0.3 to 0.6 V was applied. MEC alone resulted in a hydrogen production of 74.29 mL H_2/L and thus the overall production of hydrogen increased up to 136.59 mL H_2/L.
机译：发酵氢气的生产是在一个密封的锥形烧瓶中进行的，使用从土壤中分离出来的兼性细菌菌株，通过水置换法收集气体。在液体培养基中以玉米秸秆水解产物为碳源进行发酵。发酵结束时最大氢产量为62.3 mL H_2 / L。发酵液的HPLC分析表明存在发酵终产物，如草酸，酒石酸，甲酸，丙酮酸和柠檬酸。在第二阶段中，将具有有机酸的发酵流出物用作微生物电解池（MEC）的进料，以进一步产生氢气。通过离心将细胞与流出物分离。 MEC的阳极（A型碳布）和阴极（B型碳布）相距2 cm。 MEC的阳极富含带有废水接种物的微生物燃料电池。通过氮气10分钟后，施加0.3至0.6 V的电压。单独的MEC产生的氢气量为74.29 mL H_2 / L，因此氢气的总产量增加至136.59 mL H_2 / L。

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