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首页> 外文期刊>International Journal of Environment and Bioenergy >Effect of Ethyl Methanesulfonate on Fatty Acid Characteristic of Nannochloropsis sp.
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Effect of Ethyl Methanesulfonate on Fatty Acid Characteristic of Nannochloropsis sp.

机译：甲磺酸乙酯对拟南芥脂肪酸特征的影响。

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The ability of microalgae to grow in short time with huge biomass production has become the reference point to produce biofuel in short time as well. Until now, it still needs microalgae species that is capable of producing numerous biomass and high levels of fatty acid so it is able to supply basic needs for biofuel. One of the method used to increase microalgae biomass is mutagenesis. Mutagenesis method here is conducted by adding certain compounds called Ethyl Methanesulfonate (EMS) to microalgae cell. The results of this study showed that the highest biomass was obtained from Nannochloropsis sp. cultivation with 0.1 M EMS concentration in two different phases (1.08 ± 0.33 and 1.11 ± 0.07 gr/L). Gas chromatography identification has detected the presence of 22 compounds of saturated fatty acid and 3 compounds of unsaturated fatty acid. Three compounds of unsaturated fatty acid detected were C18:2 cis-9 (methyl oleate), C16:1 (methyl palmitoleate) and C18:2 (methyl linoleic). The highest percent area/concentration of saturated and unsaturated fatty acid respectively is C16:0 (methyl palmitate) as much as 67.70±0.05 at stationary phase and C16:1 (methyl palmitoleate) as much as 17.59±0.42 at death phase. From the results, it can be implied that EMS input in microalgae cultivation can increase the percentage of certain fatty acids that potentially produce to be biodiesel.
机译：微藻在短时间内具有大量生物质生产的能力也已成为在短时间内生产生物燃料的参考点。到目前为止，它仍然需要能够产生大量生物质和高含量脂肪酸的微藻种，因此能够满足生物燃料的基本需求。用于增加微藻生物量的方法之一是诱变。诱变方法是通过将某些称为甲磺酸乙酯（EMS）的化合物添加到微藻细胞中来进行的。这项研究的结果表明，最高的生物量是从Nannochloropsis sp。获得的。在两个不同阶段（1.08±0.33和1.11±0.07 gr / L）以0.1 M EMS浓度进行培养。气相色谱法鉴定检测到存在22种饱和脂肪酸化合物和3种不饱和脂肪酸化合物。检测到的三种不饱和脂肪酸化合物为C18：2顺式9（油酸甲酯），C16：1（棕榈油酸甲酯）和C18：2（亚油酸甲酯）。饱和和不饱和脂肪酸的最高面积/浓度百分数分别为：固定相C16：0（棕榈酸甲酯）高达67.70±0.05，死亡相C16：1（棕榈油酸甲酯）高达17.59±0.42。从结果可以暗示，在微藻培养中输入EMS可以增加某些可能产生为生物柴油的脂肪酸的百分比。

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