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首页> 外文期刊>International Journal of Electrochemical Science >Highly Stable Hydrogen Peroxide Biosensor Based on Gelatin- Hierarchical Porous Carbon Obtained from Fish Scales Modified Glassy Carbon Electrode
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Highly Stable Hydrogen Peroxide Biosensor Based on Gelatin- Hierarchical Porous Carbon Obtained from Fish Scales Modified Glassy Carbon Electrode

机译：基于鱼鳞修饰玻碳电极得到的明胶-层次多孔碳的高度稳定的过氧化氢生物传感器

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摘要

Biosensors for continuous application are becoming more important, and long-life enzyme electrodes have not been commercialized due to the lack of stability of its components. A highly stable biosensor for hydrogen peroxide (H2O2) based on gelatin-hierarchical porous carbon obtained from fish scales has been developed in this work. Catalase (Cat) immobilized on gelatin-hierarchical porous carbon was realized by the adsorption-crosslinked, here glutaraldehyde (GAD) was used as cross-linking agent. The surface morphologies of modified glass carbon electrodes(GCE) were characterized by scanning electron microscopy (SEM), and the electrochemical behaviors of the biosensor were characterized by cyclic voltammogram (CV) and electrochemical impedance spectroscopy (EIS). This biosensor exhibited a linear range from 0.2 to 10.7 mM with a correlation coefficient (R2) of 0.999, a low detection limit of 0.7 μM and a sensitivity of 74.69 μAmM-1cm-2. Especially, the stability of the as-prepared biosensor was satisfied at room temperature, which means there was almost no change in the performance even after 100 continuous scans, and the storage stability is over 30 days with retention of 98.7% activity only.
机译：连续应用的生物传感器变得越来越重要，由于其成分缺乏稳定性，长寿命酶电极尚未商业化。在这项工作中，已经开发出了一种高度稳定的过氧化氢（H2O2）生物传感器，该传感器基于从鱼鳞获得的明胶-层次多孔碳。通过吸附-交联实现了固定在明胶-分级多孔碳上的过氧化氢酶（Cat），此处戊二醛（GAD）用作交联剂。通过扫描电子显微镜（SEM）表征了修饰玻璃碳电极（GCE）的表面形貌，并通过循环伏安法（CV）和电化学阻抗谱（EIS）表征了生物传感器的电化学行为。该生物传感器的线性范围为0.2到10.7 mM，相关系数（R2）为0.999，检测下限为0.7μM，灵敏度为74.69μAmM-1cm-2。尤其是，所制备的生物传感器在室温下的稳定性令人满意，这意味着即使连续扫描100次后性能几乎也没有变化，并且储存稳定性超过30天，仅保留了98.7％的活性。

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