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首页> 外文期刊>International Journal of Electrochemical Science >Electrochemical Immunosensor for Monocyte Chemoattractant Protein-1 Detection Based on Pt Nanoparticles Functionalized Single-walled Carbon Nanohorns
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Electrochemical Immunosensor for Monocyte Chemoattractant Protein-1 Detection Based on Pt Nanoparticles Functionalized Single-walled Carbon Nanohorns

机译：基于Pt纳米粒子功能化单壁碳纳米角单核细胞趋化蛋白-1检测的电化学免疫传感器。

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摘要

A novel label-free electrochemical sensing strategy for detecting monocyte chemoattractant protein-1 (MCP-1) was developed by using single-walled carbon nanohorns (SWCNHs) functionalized with Pt nanoparticles (PtNPs-SWCNHs). After modification with the PtNPs-SWCNHs, the electrode could serve as an effective platform for antibody immobilization and electron transfer owing to the large surface area and excellent conductivity of PtNPs-SWCNHs. Moreover, the PtNPs-SWCNHs composite could provide an obvious electrochemical signal through amperometric i–t curve method, due to its high catalytic activity for the reduction of hydrogen peroxide (H2O2). Under optimal conditions, the amperometric i-t curve signals had a strong linear relation with the logarithm of the MCP-1 concentration ranging from 0.06 pg mL-1 to 450 pg mL-1, and the detection limit was 0.02 pg mL-1. Furthermore, the proposed immunosensor could detect MCP-1 in serum samples with desirable results. Overall, this immunosensor may provide a new alternative strategy for rapid detection of MCP-1.
机译：通过使用经Pt纳米粒子（PtNPs-SWCNHs）功能化的单壁碳纳米角（SWCNHs），开发了一种检测单核细胞趋化蛋白1（MCP-1）的新型无标记电化学传感策略。用PtNPs-SWCNHs修饰后，由于PtNPs-SWCNHs的大表面积和优异的电导率，该电极可作为抗体固定和电子转移的有效平台。此外，由于PtNPs-SWCNHs复合物对过氧化氢（H2O2）的还原具有高催化活性，因此可以通过安培i-t曲线方法提供明显的电化学信号。在最佳条件下，安培i-t曲线信号与MCP-1浓度的对数之间的线性关系很强，范围为0.06 pg mL-1至450 pg mL-1，检出限为0.02 pg mL-1。此外，所提出的免疫传感器可以检测血清样品中的MCP-1，具有理想的结果。总体而言，该免疫传感器可能为MCP-1的快速检测提供新的替代策略。

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