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首页> 外文期刊>International Journal of Electrochemical Science >Electrochemical and Spectroscopic Study on the Interaction Between G-Quadruplex DNA and (R)-/(S)-2-(5-Fluorouracil-1-Acetyl) Amido-1, 5-Dimethyl Glutarate
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Electrochemical and Spectroscopic Study on the Interaction Between G-Quadruplex DNA and (R)-/(S)-2-(5-Fluorouracil-1-Acetyl) Amido-1, 5-Dimethyl Glutarate

机译：G-四链体DNA与（R）-/（S）-2-（5-氟尿嘧啶-1-乙酰基）氨基1，戊二酸5-二甲酯相互作用的电化学和光谱研究

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The cyclic voltammetry and ultraviolet-visible absorption spectrometry have proved to be powerfulelectrochemical means for the research of interactions between G-quadruplex DNA (G4-DNA) andchemical anti-carcinogens. In this study, the electrochemical behavior of (R)-/(S)-2-(5-fluorouracil-1- acetyl) amido-1,5-dimethyl glutarate (i.e., (R)-5FUGlu and (S)-5FUGlu) and the interaction of (R)- 3-/4- /(S)-2-(5-fluorouracil-1-acetyl) amido-1,5-dimethyl glutarate with DNA using Fe(CN)6 aselectroactive indicator were investigated, and the compounds were assayed for anticancer activities inHL-60 cells. The experiment showed that the interaction between (R)-/(S)-2-(5-fluorouracil-1- acetyl)amido-1,5-dimethyl glutarate and DNA were electrostatic, and the binding equilibrium constantKG4-DNA of the compounds is closely related to the configuration. It was observed that the bindingequilibrium constant KG4-DNA of (R)-5FUGlu was about 2 times larger than that of (S)-5FUGlu. Theinhibition rates of the different concentration of (R)-5FUGlu in tumor cell line HL-60 cells were largerthan those of (S)-5FUGlu, which gave a good agreement with the above-mentioned electrochemicalresults.
机译：循环伏安法和紫外可见吸收光谱法已被证明是研究G四联体DNA（G4-DNA）与化学抗癌剂之间相互作用的强大电化学手段。在这项研究中，（R）-/（S）-2-（5-氟尿嘧啶-1-乙酰基）氨基1,5-戊二酸戊二酯的电化学行为（即（R）-5FUGlu和（S）-5FUGlu ）并使用Fe（CN）6作为电活性指示剂研究了（R）-3- / 4-/（S）-2-（5-氟尿嘧啶-1-乙酰基）戊基1,5,5-二甲基戊二酸与DNA的相互作用，并测定了该化合物在HL-60细胞中的抗癌活性。实验表明，（R）-/（S）-2-（5-氟尿嘧啶-1-乙酰基）酰氨基-1,5-二甲基戊二酸与DNA的相互作用是静电的，化合物的结合平衡常数KG4-DNA与配置密切相关。观察到，（R）-5FUGlu的结合平衡常数KG4-DNA是（S）-5FUGlu的约2倍。肿瘤细胞系HL-60细胞中不同浓度的（R）-5FUGlu的抑制率大于（S）-5FUGlu，与上述电化学结果吻合良好。

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