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首页> 外文期刊>International Journal of Electrochemical Science >A Biosensor for Hydrogen Peroxide Based on Single walled Carbon Nanotube and Metal Oxide Modified Indium Tin Oxide Electrode
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A Biosensor for Hydrogen Peroxide Based on Single walled Carbon Nanotube and Metal Oxide Modified Indium Tin Oxide Electrode

机译：基于单壁碳纳米管和金属氧化物修饰的铟锡氧化物电极的过氧化氢生物传感器

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A novel approach has been developed for a highly sensitive, fast responding and stablemonoenzymatic amperometric biosensor for the detection of hydrogen peroxide using coimmobilizedthe single walled carbon nanotube (SWNT) and methylene blue (MB) into the metal oxide (SnO2)derived organically modified sol-gel glasses (ormosil). Nafion (NAF) was dispersed within the ormosilmatrix to enhance the electron transportation in the modified film. Horseradish peroxidase (HRP) wasdiffusionally adsorbed onto the SWNT/ormosil modified ITO (Indium tin oxide) electrode surface. TheSWNT/ormosil-modified electrodes were characterized with scanning electron microscopy (SEM),Atomic Force microscopy (AFM) and UV-vis spectroscopy. Cyclic voltammetry and amperometrymeasurements were used to study and optimize the performance of the resulting peroxide biosensor.The apparent Michaelis-Menten constant was determined to be 1.6mM. The effect of pH, SWNTcontent, applied potential and temperature on the peroxide biosensor has been systemically studied.The fabricated biosensor had a fast response of H2O2, less than 5s and excellent linear range of-7 -4 concentration from 5x10 to 1x10 M with the detection limit of 0.3M (S/N=3) under the optimumconditions. These results show that this amperometric biosensor could be used for the measurement ofH2O2 in cellular, enzyme systems or cancer cell studies without the help of any fluorescent probes ordetection reagents.
机译：已开发出一种新方法，用于将单壁碳纳米管（SWNT）和亚甲基蓝（MB）共同固定在金属氧化物（SnO2）衍生的有机改性溶胶-中，用于检测过氧化氢的高灵敏度，快速响应和稳定的单酶安培生物传感器。凝胶眼镜（ormosil）。 Nafion（NAF）分散在ormosilmatrix中，以增强改性膜中的电子传输。辣根过氧化物酶（HRP）扩散吸附到SWNT / ormosil改性的ITO（铟锡氧化物）电极表面。通过扫描电子显微镜（SEM），原子力显微镜（AFM）和紫外可见光谱对SWNT / ormosil修饰的电极进行表征。使用循环伏安法和安培法研究并优化了所得过氧化物生物传感器的性能，表观Michaelis-Menten常数确定为1.6mM。 pH值，SWNT含量，施加电势和温度对过氧化物生物传感器的影响已得到系统研究。所制备的生物传感器具有H2O2的快速响应，不到5s的时间和良好的线性范围（-7 -4浓度从5x10到1x10 M）。最佳条件下的检出限为0.3M（S / N = 3）。这些结果表明，这种电流型生物传感器无需任何荧光探针或检测试剂即可用于细胞，酶系统或癌细胞研究中的H2O2测量。

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