Amperometric and Impedimetric H<sub>2</sub>O<sub>2</sub> Biosensor Based on Horseradish Peroxidase Covalently Immobilized at Ruthenium Oxide Nanoparticles Modified Electrode

Arun Prakash Periasamy; Shan Wei Ting; Shen-Ming Chen

首页> 外文期刊>International Journal of Electrochemical Science >Amperometric and Impedimetric H₂O₂ Biosensor Based on Horseradish Peroxidase Covalently Immobilized at Ruthenium Oxide Nanoparticles Modified Electrode

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Amperometric and Impedimetric H₂O₂ Biosensor Based on Horseradish Peroxidase Covalently Immobilized at Ruthenium Oxide Nanoparticles Modified Electrode

机译：基于辣根过氧化物酶共价固定在氧化钌纳米粒子修饰电极上的电流和阻抗H _{2 O _{2 生物传感器}}

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In this work, Ruthenium oxide nanoparticles (RuNPs) were electrochemically deposited on a glassycarbon electrode (GCE) surface using RuCl3. nH2O as a precursor. The model redox enzyme,horseradish peroxidase (HRP) was covalently immobilized at the RuNPs modified electrode surfaceusing Chitosanlutaraldehyde (ChiAD) cross-linking. The surface morphology of RuNPs, HRPand HRP/ChiAD/RuNPs films were investigated using scanning electron microscopy (SEM) andatomic force microscopy (AFM) studies. From the SEM and AFM results the average size of theRuNPs was calculated as 6060 nm. UV-visible absorption spectra of HRP and HRP/Chi.

机译：在这项工作中，使用RuCl3将氧化钌纳米颗粒（RuNPs）电化学沉积在玻璃碳电极（GCE）表面上。 nH2O为前体。使用壳聚糖-戊二醛（ChiAD）交联将模型氧化还原酶，辣根过氧化物酶（HRP）共价固定在RuNPs修饰的电极表面。使用扫描电子显微镜（SEM）和原子力显微镜（AFM）研究了RuNPs，HRP和HRP / ChiAD / RuNPs薄膜的表面形态。根据SEM和AFM结果，RuNP的平均尺寸被计算为6060nm。 HRP和HRP / Chi的紫外可见吸收光谱。

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《International Journal of Electrochemical Science》 |2011年第7期|共22页
作者
Arun Prakash Periasamy; Shan Wei Ting; Shen-Ming Chen;
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Amperometric and Impedimetric H2O2 Biosensor Based on Horseradish Peroxidase Covalently Immobilized at Ruthenium Oxide Nanoparticles Modified Electrode

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Amperometric and Impedimetric H₂O₂ Biosensor Based on Horseradish Peroxidase Covalently Immobilized at Ruthenium Oxide Nanoparticles Modified Electrode