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首页> 外文期刊>International Journal of Electrochemical Science >Spectroelectrochemical Dynamics of Dendritic Poly (Propylene imine)-Polythiophene Star Copolymer Aptameric 17B-Estradiol Biosensor
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Spectroelectrochemical Dynamics of Dendritic Poly (Propylene imine)-Polythiophene Star Copolymer Aptameric 17B-Estradiol Biosensor

机译：树突状聚（丙烯亚胺）-聚噻吩星型共聚物Apameric 17B-雌二醇生物传感器的光谱电化学动力学

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Aptamers, which are in vitro-selected functional oligonucleotides, have been employed to design novelaptasensor due to their inherent high selectivity and affinity compared to traditional biorecognitionelements. This report presents a novel aptamer biosensor for determining the endocrine disruptingcompound (EDC), 17-estradiol (E2), which was constructed from a SELEX-synthesized 76-merbiotinylated aptamer for 17estradiol incorporated in a dendritic generation 1 poly(propylene imine)- polythiophene (G1PPT-co-PEDOT) star copolymer-functionalised Au electrode via biotin-avidininteraction. The sensor platform and aptasensor were interrogated with scanning electron microscopy(SEM), FTIR, electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) and squarewave voltammetry (SWV). The kinetic parameters of the sensor platform were determined by-3/-4 modelling the [Fe(CN)6] (redox probe) Nyquist and Bode impedimetric spectra to the appropriateequivalent electrical circuit. The EIS spectra shows that at low frequencies (100 mHz) when theelectronics of the electrode systems are only minimally perturbed the Au糋1PPT-co-PEDOTnanoelectrode exhibited greater semi-conductor behaviour (higher phase angle value) than Au糋1PPTdue to the incorporation of charged functionalized dendrimer. However, the Bode plot also shows thatthe charge transfer dynamics of the nanoelectrode can be frequency modulated. The biosensorresponse to 17-estradiol was based on the decrease in the SWV current as the EDC binds to thessDNA aptamer on the biosensor. The dynamic linear range of the sensor was 0.1 100 nM. Theseinitial studies also showed that the aptamer used in this study was very selective to, and reproduciblefor, 17--estradiol.
机译：作为体外选择的功能性寡核苷酸的适体，由于其与传统生物识别元件相比固有的高选择性和亲和力，已被用于设计新型适体传感器。本报告介绍了一种新型的适体生物传感器，用于确定内分泌干扰化合物（EDC），17-雌二醇（E2），该传感器由SELEX合成的76-生物素化的17-雌二醇适体构建，并掺入了第1代树突状聚（丙烯亚胺）-聚噻吩中（G1PPT-co-PEDOT）通过生物素-亲和素相互作用将星形共聚物官能化的金电极。通过扫描电子显微镜（SEM），FTIR，电化学阻抗谱（EIS），循环伏安法（CV）和方波伏安法（SWV）对传感器平台和适体传感器进行了研究。传感器平台的动力学参数是通过将[Fe（CN）6]（氧化还原探针）Nyquist和Bode阻抗谱建模为适当的等效电路的3-/-4来确定的。 EIS光谱表明，在低频（100 mHz）下，当电极系统的电子仅受到最小干扰时，由于掺入了Au糋1PPT，纳米电极表现出比Au糋1PPT更大的半导体行为（更高的相角值）。带电的功能化树枝状聚合物。然而，伯德图还表明，可以对纳米电极的电荷转移动力学进行频率调制。生物传感器对17-雌二醇的反应是基于当EDC与生物传感器上的ssDNA适体结合时SWV电流的减少。传感器的动态线性范围为0.1 100 nM。这些初始研究还表明，本研究中使用的适体对17-雌二醇具有很高的选择性，并且可重现。

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