• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>International Journal of Electrochemical Science >Using of Paramagnetic Microparticles and Quantum Dots for Isolation and Electrochemical Detection of Influenza Viruses’ Specific Nucleic Acids
【24h】

Using of Paramagnetic Microparticles and Quantum Dots for Isolation and Electrochemical Detection of Influenza Viruses’ Specific Nucleic Acids

机译：利用顺磁性微粒和量子点分离和电化学检测流感病毒的特定核酸

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The aim of this study was to suggest isolation protocol based on paramagnetic microparticles andmulticolour electrochemical detection of three various influenza oligonucleotides (H1N1 influenzasubtype, H3N2 influenza subtype and H5N1 influenza subtype) modified with nanoparticles (NPs)called quantum dots (QDs), which are made from zinc, cadmium or lead. Particularly, we describedhybridization assay based on paramagnetic microparticles (MPs) and coupled with detection of threedifferent influenza derived oligonucleotides (ODNs) labelled with QDs. Hybridization efficiencybetween NPs conjugated anti-senses and target nucleic acid hybrids was optimized and evaluated bythe electrochemical analysis. The highest response of ODN-SH-NPs was determined for thehybridization temperature of 25 C. Further, we tested detection of the complexes via peak comingfrom the presence of ODNs and/or from the presence of metal ions in QDs. Under the optimizedconditions, LODs (3 S/N) based on the determination of metal ions in QDs label were estimated asfollows: 0.1 ng/ml of ODN-SH-CdNPs, 0.5 ng/ml of ODN-SH-ZnNPs and 1 ng/ml of ODN-SH- PbNPs. Besides this we also correlated the results from ODNs and metal ions signals.
机译：这项研究的目的是提出一种基于顺磁性微粒的分离方案，并通过三种被称为量子点（QD）的纳米粒子修饰的流感病毒寡核苷酸（H1N1流感亚型，H3N2流感亚型和H5N1流感亚型）进行多色电化学检测来自锌，镉或铅。特别是，我们描述了基于顺磁性微粒（MPs）的杂交测定，并结合了用QD标记的三种不同流感衍生寡核苷酸（ODN）的检测。通过电化学分析优化并评价了NPs结合的反义核酸与靶核酸杂交体之间的杂交效率。在25°C的杂交温度下，确定了ODN-SH-NPs的最高响应。此外，我们通过QDs中存在ODNs和/或金属离子的峰检测了络合物的检测。在优化的条件下，根据QDs标签中金属离子的测定，LODs（3 S / N）估计如下：ODN-SH-CdNPs 0.1 ng / ml，ODN-SH-ZnNPs 0.5 ng / ml和1 ng / ml ml的ODN-SH- PbNPs。除此之外，我们还关联了ODN和金属离子信号的结果。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号