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首页> 外文期刊>International Journal of Electrochemical Science >Chemiluminescent Detection of DNA Hybridization Based on Signal DNA Probe Modified with Gold and Cobalt Nanoparticles
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Chemiluminescent Detection of DNA Hybridization Based on Signal DNA Probe Modified with Gold and Cobalt Nanoparticles

机译：基于金和钴纳米粒子修饰的信号DNA探针的DNA杂交化学发光检测

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摘要

A novel and sensitive chemiluminescence (CL) assay for sequence-specific DNA detection based onsignal amplification with gold and cobalt nanoparticles (NPs) was reported. The sandwich-type DNAbiosensor was fabricated with the amino-functionalized capture DNA immobilized on the magneticbead and hybridized with one end of target DNA, the other end of which was recognized with a signalDNA probe labeled on the surface of Au NPs. To amplify the detection signals, a single gold NP wasmodified with 27 cobalt NPs by a reaction between amino-functionalized DNA on the surface of goldNP and carboxyl-functionalized on the surface of cobalt NPs. The hybridization events were monitored2+ by the CL intensity of luminol-H2O2-Co after the cobalt ions were dissolved from the hybrids. This-17 -16 method could detect as low as 6.0 10 M target DNA and the line range was from 1.0 10 to 1.0-15 10 M.
机译：报道了一种新的灵敏的化学发光（CL）检测法，用于基于金和钴纳米粒子（NP）的信号扩增的序列特异性DNA检测。三明治型DNA生物传感器的制备是将氨基官能化的捕获DNA固定在磁珠上，并与目标DNA的一端杂交，另一端被标记在Au NPs表面的信号DNA探针识别。为了放大检测信号，通过在goldNP表面的氨基官能化DNA和在钴NPs表面的羧基官能化的反应，用27个钴NP修饰单个金NP。钴离子从杂种中溶解后，通过luminol-H2O2-Co的CL强度监控杂交事件。此17 -16方法可检测到低至6.0 10 M的目标DNA，且行范围为1.0 10至1.0-15 10M。

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