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Fabrication of a Highly Sensitive Electrochemical Immunosensor for Human Epididymis Protein 4 (HE4) Detection

机译:用于人类附睾蛋白4(HE4)检测的高灵敏度电化学免疫传感器的制备。

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Diagnostics of ovarian cancer can be achieved by detecting the level of human epididymis protein 4(HE4). The present study proposed the preparation of a biosensor based on a nitrogen-dopedgraphene/gold nanoparticle (AuNP)/fluorine-doped tin oxide (FTO) glass electrode modified withfructosyl amino-acid oxidase (FAO), i.e., the AuNPs/N-doped GNs/FTO. The proposed electrode wasfound to be highly sensitive and selective towards the biosensing of HE4, with a the limit of detection(LOD) calculated at 1.21 μg/mL and a linear detection range of 10 to 65 μg/mL.
机译:卵巢癌的诊断可以通过检测人附睾蛋白4(HE4)的水平来实现。本研究提出了基于果糖基氨基酸氧化酶(FAO)修饰的氮掺杂石墨烯/金纳米颗粒(AuNP)/氟掺杂氧化锡(FTO)玻璃电极的生物传感器的制备,即AuNPs / N掺杂GNs / FTO。发现拟议的电极对HE4的生物传感具有高度的敏感性和选择性,计算的检测极限(LOD)为1.21μg/ mL,线性检测范围为10至65μg/ mL。

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