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首页> 外文期刊>International Journal of Electrochemical Science >Electrochemical Quantification of CK-MB in Serum Based on Immunoassay
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Electrochemical Quantification of CK-MB in Serum Based on Immunoassay

机译：基于免疫测定的血清中CK-MB的电化学定量

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摘要

Creatine kinase isoenzyme (CK-MB) is a cardiac biomarker for post-PCI recovery evaluation. This work describes a novel biosensor concept that can be used to quantify enzyme levels where the substrate is immobilized directly on the glassy carbon electrode (GCE). The phosphorylated form of creatine (PC) was immobilized on the electrode with cysteamine (Cys) by a self-assembling monolayer technique. Electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) studies were used to follow the chemical modification process. The proposed electrochemical sensor could detect MB-CK at a range from 0.1 to 2000 ng/mL. The detection limit was determined to be 0.04 ng/mL.
机译：肌酸激酶同工酶（CK-MB）是用于PCI后评估的心脏生物标志物。这项工作描述了一种新颖的生物传感器概念，可用于定量将底物直接固定在玻璃碳电极（GCE）上的酶水平。通过自组装单层技术，将磷酸肌酸（PC）的形式与半胱胺（Cys）固定在电极上。电化学阻抗谱（EIS）和循环伏安法（CV）研究用于跟踪化学修饰过程。拟议的电化学传感器可以检测到0.1-2000 ng / mL范围内的MB-CK。检出限确定为0.04 ng / mL。

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