• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>International Journal of Electrochemical Science >A Sensitive Electrochemical Impedance DNA Biosensor Based on ZnO Nanorod Electrodes for BCR/ABL Fusion Gene Detection
【24h】

A Sensitive Electrochemical Impedance DNA Biosensor Based on ZnO Nanorod Electrodes for BCR/ABL Fusion Gene Detection

机译：基于ZnO纳米棒电极的敏感电化学阻抗DNA生物传感器用于BCR / ABL融合基因检测。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A ZnO based DNA biosensor was developed to detect complementary target single-stranded DNA (ssDNA)of BCR/ABL fusion gene. ZnO nanorods (NRs) have been synthesized onto platinized silicon (Pt/Si) substrateusing facile and economical chemical bath deposition method. The ssDNA probe was immobilized byelectrostatic attraction on the ZnO/Pt/Si surface. The morphology of the samples were characterized byscanning electron microscopy. Electrochemical impedance spectroscopy and cyclic voltammetry were doneto study ZnO NR arrays, and the immobilization and the hybridization of ssDNA at the electrode surface. Thefabricated DNA biosensor was evaluated in the complementary target ssDNA of BCR/ABL probe in thedynamic range from 1.0 pM/L to 1.0 nM/L which indicates a good linearity, high sensitivity and low detectionlimit of 2.75 × 10 ?13 mol/L. The successful detection of BCR/ABL fusion gene exhibits its potential to be arobust and sensitive label-free electrochemical impedance measurement for sensing applications.
机译：基于ZnO的DNA生物传感器被开发来检测BCR / ABL融合基因的互补目标单链DNA（ssDNA）。 ZnO纳米棒（NRs）已使用简便且经济的化学浴沉积方法合成到了镀铂硅（Pt / Si）衬底上。通过静电吸引将ssDNA探针固定在ZnO / Pt / Si表面上。通过扫描电子显微镜表征样品的形态。进行了电化学阻抗谱和循环伏安法研究ZnO NR阵列，以及ssDNA在电极表面的固定和杂交。在BCR / ABL探针的互补目标ssDNA中对合成的DNA生物传感器进行了评估，其动态范围为1.0 pM / L至1.0 nM / L，这表明线性度好，灵敏度高且检测限低至2.75×10？13 mol / L。 BCR / ABL融合基因的成功检测显示出其潜在的鲁棒性和灵敏的无标记电化学阻抗测量的传感应用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号