y-Fe<sub>2</sub>O<sub>3</sub> Magnetic Core Functionalized with Tetraethyl Orthosilicate and 3-Aminopropyl Triethoxysilane for an Isolation of H7N7 Influenza Serotype Virions

Zbynek Heger; Natalia Cernei; Roman Guran; Petr Michalek; Vedran Milosavljevic; Pavel Kopel; Ondrej Zitka; Jindrich Kynicky; Petr Lany; Vojtech Adam; Rene Kizek

首页> 外文期刊>International Journal of Electrochemical Science >y-Fe₂O₃ Magnetic Core Functionalized with Tetraethyl Orthosilicate and 3-Aminopropyl Triethoxysilane for an Isolation of H7N7 Influenza Serotype Virions

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y-Fe₂O₃ Magnetic Core Functionalized with Tetraethyl Orthosilicate and 3-Aminopropyl Triethoxysilane for an Isolation of H7N7 Influenza Serotype Virions

机译：用原硅酸四乙酯和3-氨基丙基三乙氧基硅烷官能化的y-Fe _{2 O _{3 磁芯用于分离H7N7流感病毒毒粒}}

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The influenza viruses cause annual epidemics of respiratory disease and occasional pandemics, whichconstitute a major public-health issue. The most important tool in the diagnosis of influenza pandemicis timely and right diagnosis. Hence, this study presents synthesis of paramagnetic particles formed bynanomaghemite (-Fe2O3) core, whose surface was functionalized with tetraethyl orthosilicate (TEOS)and 3-aminopropyl triethoxysilane (APTES), able to bind H7N7 influenza virions. Paramagneticparticles show the ability to immobilize virions for direct analysis using ion-exchange chromatographywith VIS detection. The presence of H7N7 virions on surface of paramagnetic beads was confirmed bymatrix-assisted laser desorption/ionization time of flight mass spectrometry, based upon the massweight of H7N7 virions, determined before and after establishment of binding. Moreover, usingscanning electrochemical microscopy revealed, that binding is provided due to strong charge-chargeinteraction between positively charged influenza surface protein hemagglutinin and negatively chargedsurface of nanocomposite. It can be concluded that paramagnetic beads show significant bindingcapability towards H7N7 virions.

机译：流感病毒每年引起呼吸道疾病的流行和偶发的大流行，这构成了主要的公共卫生问题。及时，正确地诊断流感大流行的最重要工具。因此，本研究提出了由纳米磁铁矿（-Fe2O3）核形成的顺磁性粒子的合成方法，其表面被原硅酸四乙酯（TEOS）和3-氨基丙基三乙氧基硅烷（APTES）官能化，能够结合H7N7流感病毒粒子。顺磁性粒子显示了使用离子交换色谱和VIS检测将病毒粒子固定化以进行直接分析的能力。通过在建立结合之前和之后确定的H7N7病毒粒子的质量，通过基质辅助激光解吸/电离飞行时间质谱法确认顺磁性珠粒表面上存在H7N7病毒粒子。此外，使用扫描电化学显微镜揭示，由于带正电荷的流感表面蛋白血凝素与带负电荷的纳米复合材料表面之间的强电荷-电荷相互作用而提供了结合。可以得出结论，顺磁珠显示出对H7N7病毒体的显着结合能力。

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《International Journal of Electrochemical Science》 |2014年第7期|共12页
作者
Zbynek Heger; Natalia Cernei; Roman Guran; Petr Michalek; Vedran Milosavljevic; Pavel Kopel; Ondrej Zitka; Jindrich Kynicky; Petr Lany; Vojtech Adam; Rene Kizek;
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1. y-Fe₂O₃ Magnetic Core Functionalized with Tetraethyl Orthosilicate and 3-Aminopropyl Triethoxysilane for an Isolation of H7N7 Influenza Serotype Virions [J] . Zbynek Heger, Natalia Cernei, Roman Guran, International Journal of Electrochemical Science . 2014,第7期

机译：用原硅酸四乙酯和3-氨基丙基三乙氧基硅烷官能化的y-Fe _{2 O _{3 磁芯用于分离H7N7流感病毒毒粒}}
2. A two-step protocol for isolation of influenza A (H7N7) virions and their RNA for PCR diagnostics based on modified paramagnetic particles [J] . Michalek Petr, Dostalova Simona, Buchtelova Hana, Electrophoresis: The Official Journal of the International Electrophoresis Society . 2016,第14期

机译：基于修饰的顺磁粒子的两步协议，用于分离甲型流感病毒（H7N7）及其RNA用于PCR诊断
3. Bioinspired, Cysteamine-Catalyzed Co-Silicification of (1H, 1H, 2H, 2H- Perfluorooctyl)triethoxysilane and Tetraethyl Orthosilicate: Formation of Superhydrophobic Surfaces [J] . Ji Hun Park, Ji Yup Kim, Woo Kyung Cho, Chemistry, an Asian journal . 2014,第3期

机译：生物启发的半胱胺催化的（1H，1H，2H，2H-全氟辛基）三乙氧基硅烷和原硅酸四乙酯的共硅化：超疏水表面的形成
4. Direct functionalization of magnetic hollow spheres with (3-aminopropyl)triethoxysilane (APTES) for targeted drug delivery [C] . P.B. Patil, V.C. Karade, P.P. Waifalkar, 2017 IEEE International Magnetics Conference . 2017

机译：用（3-氨基丙基）三乙氧基硅烷（APTES）对磁性空心球进行直接功能化以实现靶向药物递送
5. Mossbauer, Electron Paramagnetic Resonance, and Density Functional Theory Studies of Biomimetric Iron(IV)=Oxygen Species and High-Valent Diamond Core Intermediates. [D] . De Hont, Raymond F. 2010

机译：仿生铁（IV）=氧物种和高价金刚石核中间体的Mossbauer，电子顺磁共振和密度泛函理论研究。
6. A sonochemical approach to the direct surface functionalization of superparamagnetic iron oxide nanoparticles with (3-aminopropyl)triethoxysilane [O] . Bashiru Kayode Sodipo, Azlan Abdul Aziz 2014

机译：超声化学方法研究超顺磁性氧化铁纳米粒子与（3-氨丙基）三乙氧基硅烷的直接表面官能化
7. A sonochemical approach to the direct surface functionalization of superparamagnetic iron oxide nanoparticles with (3-aminopropyl)triethoxysilane [O] . Bashiru Kayode Sodipo, Azlan Abdul Aziz 2014

机译：超顺磁性氧化铁纳米粒子与（3-氨基丙基）三乙氧基硅烷直接表面官能化的声化学方法

y-Fe2O3 Magnetic Core Functionalized with Tetraethyl Orthosilicate and 3-Aminopropyl Triethoxysilane for an Isolation of H7N7 Influenza Serotype Virions

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y-Fe₂O₃ Magnetic Core Functionalized with Tetraethyl Orthosilicate and 3-Aminopropyl Triethoxysilane for an Isolation of H7N7 Influenza Serotype Virions