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首页> 外文期刊>International Journal of Electrochemical Science >Direct Electron Transfer of Glucose Oxidase in Carbon Paper for Biofuel Cells and Biosensors
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Direct Electron Transfer of Glucose Oxidase in Carbon Paper for Biofuel Cells and Biosensors

机译：用于生物燃料电池和生物传感器的复写纸中葡萄糖氧化酶的直接电子转移

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Advanced bioelectronic devices, such as high-power biofuel cells (BFCs) and highly efficient biosensors, are limited by the difficulty of electron transfer between enzymes and electrodes. Previously reported methods for achieving electron transfer from enzymes to electrodes have relied on the use of complex biomolecule immobilization procedures, complicated matrix materials, or enzyme engineering, resulting in potential relative toxicity, high cost, as well as limited stability. Here, we report a facile method for the rapid preparation of a glucose oxidase (GOx) anode with direct electron transfer (DET) for glucose BFCs and biosensors. GOx is directly incorporated into pretreated carbon paper (CP) by adjusting the pH of the incubation medium during the immobilization process. Eexcellent bioelectrocatalytic activity is obtained when GOx is incorporated into CP near the pI of GOx. The electron transfer rate constant ( ) and the apparent Michaelis-Menten constant ( ) are estimated to be 12.08 ± 1.0 s-1 and 0.13 ± 0.01 mM, respectively. These findings may be extended to the development of highly conductive nanomaterials and the immobilization of other enzymes or biomolecules, providing a promising platform for the development of BFCs, biosensors, and other bioelectrochemical devices.
机译：诸如大功率生物燃料电池（BFC）和高效生物传感器之类的先进生物电子设备受到酶和电极之间电子转移的困难的限制。先前报道的用于实现电子从酶到电极的转移的方法依赖于使用复杂的生物分子固定程序，复杂的基质材料或酶工程，从而导致潜在的相对毒性，高成本以及有限的稳定性。在这里，我们报告一种简便的方法，用于葡萄糖BFC和生物传感器的快速制备具有直接电子转移（DET）的葡萄糖氧化酶（GOx）阳极。通过在固定过程中调节培养介质的pH值，将GOx直接掺入预处理的碳纸（CP）中。当GOx在GOx的pI附近掺入CP时，可获得出色的生物电催化活性。电子传输速率常数（）和表观米氏（Michaelis-Menten）常数（）分别估计为12.08±1.0 s-1和0.13±0.01 mM。这些发现可能会扩展到高导电性纳米材料的开发以及其他酶或生物分子的固定化，为BFC，生物传感器和其他生物电化学装置的开发提供了有希望的平台。

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