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首页> 外文期刊>International Journal of Electrochemical Science >Amperometric Phenol Biosensor Based on a New Immobilization Matrix: Polypyrrole Nanotubes Derived from Methyl Orange as Dopant
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Amperometric Phenol Biosensor Based on a New Immobilization Matrix: Polypyrrole Nanotubes Derived from Methyl Orange as Dopant

机译：基于新型固定化基质的安培苯酚生物传感器：以甲基橙为掺杂剂的聚吡咯纳米管

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摘要

A novel polypyrrole nanotubes (PPy-NTs) derived from methyl orange (MO) was used as matrix to construct a highly responsive phenol biosensor. The phenol sensor based on polyphenol oxidase could be easily obtained by casting the bio-composite via the cross-linking in the presence of glutaraldehyde on glassy carbon electrode surface. PPy-NTs matrix has features of special three-dimensional structure, biocompatible properties and high surface area, and the features above resulted in high enzyme immobilization and the enzyme buried in PPy-NTs retained its activity to a large extent. This biosensor exhibited a series of better performance such as high sensitivity (2981 mA·M-1·cm-2), good affinity to its substrate (the apparent Michaelis-Menten constant was 0.12 mM) and remarkable long- term stability (it retained 88% of the original activity after 30 days) and acceptable repeatability. The detection limit of the biosensor was 1.22 nM. Furthermore, the optimization of biosensor preparation and effects of experimental variables, such as pH, temperature and potential of the sensor were discussed.
机译：以甲基橙（MO）衍生的新型聚吡咯纳米管（PPy-NTs）为基质，构建了高响应酚生物传感器。在戊二醛存在下，通过在玻碳电极表面上通过交联来铸造生物复合材料，可以轻松获得基于多酚氧化酶的酚传感器。 PPy-NTs基质具有特殊的三维结构，生物相容性和高比表面积的特征，上述特征导致酶高度固定化，PPy-NTs中埋藏的酶在很大程度上保留了其活性。这种生物传感器表现出一系列更好的性能，例如高灵敏度（2981 mA·M-1·cm-2），与其底物的亲和力（表观Michaelis-Menten常数为0.12 mM）和出色的长期稳定性（保留下来） 30天后原始活性的88％）和可接受的重复性。生物传感器的检测限为1.22 nM。此外，还讨论了生物传感器制备的优化和实验变量（例如pH，温度和传感器电势）的影响。

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