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首页> 外文期刊>International Journal of Electrochemical Science >Highly Sensitive Electrochemical Biosensor Assembled by Au Nanoparticle /MOF-5 Composite Electrode for DNA Detection
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Highly Sensitive Electrochemical Biosensor Assembled by Au Nanoparticle /MOF-5 Composite Electrode for DNA Detection

机译：金纳米粒子/ MOF-5复合电极组装的高灵敏度电化学生物传感器，用于DNA检测

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摘要

An efficient and sensitive electrochemical method for detecting trace amounts of sequence-specificdeoxyribonucleic acid (DNA) by using a gold nanoparticle (Au NP)/metal–organic framework (MOF)composite electrode was developed. An electrochemical DNA sensor was constructed by introducing AuNPs as electrochemical signal labels to an MOF-5-modified glassy carbon electrode as the adsorptionbackground platform. The unique porous structural characteristics of MOF-5 considerably enhanced theelectrocatalytic activity of the Au NPs and improved the DNA detection limit of the resultant electrode.The cyclic voltammetry, linear sweep voltammetry, and chronoamperometry results for theelectrochemical DNA sensor demonstrated that the electro-oxidation current gradually increased as thetarget DNA concentration was increased. Target DNA concentrations of 1 pM to 100 nM presented agood linear relationship with current. The calculated detection limit was 0.05 pM. The Au/MOFcomposite electrode materials presented considerable potential for application in the development ofelectrochemical biosensors for DNA detection.
机译：开发了一种高效灵敏的电化学方法，通过使用金纳米粒子（Au NP）/金属-有机骨架（MOF）复合电极检测痕量的序列特异性脱氧核糖核酸（DNA）。通过将作为电化学信号标记的AuNPs引入作为吸附背景平台的MOF-5修饰玻碳电极上，构建了电化学DNA传感器。 MOF-5独特的多孔结构特性大大增强了金纳米颗粒的电催化活性并提高了所得电极的DNA检测极限。电化学DNA传感器的循环伏安法，线性扫描伏安法和计时安培法结果表明，电氧化电流随着靶DNA浓度的增加逐渐增加。 1 pM至100 nM的目标DNA浓度与电流呈良好的线性关系。计算的检测极限为0.05 pM。 Au / MOF复合电极材料在用于DNA检测的电化学生物传感器的开发中具有巨大的应用潜力。

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