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首页> 外文期刊>International Journal of Electrochemical Science >A Biosensor for Detect Nitrite (NO2-) and Hydroxylamine (NH2OH) by Using of Hydroxylamine Oxidase and Modified Electrode with ZnO Nanoparticles
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A Biosensor for Detect Nitrite (NO2-) and Hydroxylamine (NH2OH) by Using of Hydroxylamine Oxidase and Modified Electrode with ZnO Nanoparticles

机译：羟胺氧化酶和ZnO纳米粒子修饰电极检测亚硝酸盐（NO 2 -）和羟胺（NH 2 OH）的生物传感器

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摘要

The direct electrochemistry of Hydroxylamine oxidase (HAO) / zinc oxide (Zno) nanoparticles (Nps)- /carbon paste electrode (CPE) was studied for design a new biosensor for detect Nitrite (NO2 ) andhydroxylamine (NH2OH). A conventional three electrode cell was employed throughout theexperiments, with bare or Zno nanoparticles/ HAO modified carbon paste electrode (4.0 mm diameter)as a working electrode, an Ag/AgCl as a reference electrode and a platinum electrode as a counterelectrode; all electrochemical study done by voltammetric method ( here cyclic voltammetry (CV) ).Samples were measured and recorded using a TU-1901 double-beam UV-visible (UV-VIS)spectrophotometer and phase characterization was performed by means of X-ray diffraction (XRD)and also the morphologies and particle sizes of the samples were characterized by scanning electronmicroscopy (SEM). The results showed that the redox process was an adsorption-controlled and the- immobilized Hydroxylamine oxidase was stable and could detect NO2 concentration in solution in therange of 5 to 43 M; also mentioned biosensor could detect hydroxylamine at surface of modified CPEwith Zno (Nps).
机译：研究了羟胺氧化酶（HAO）/氧化锌（Zno）纳米颗粒（Nps）-/碳糊电极（CPE）的直接电化学，以设计用于检测亚硝酸盐（NO2）和羟胺（NH2OH）的新型生物传感器。在整个实验中使用了传统的三电极电池，裸露的或Zno纳米粒子/ HAO改性碳糊电极（直径为4.0 mm）作为工作电极，Ag / AgCl作为参比电极，铂电极作为对电极。所有的电化学研究都是通过伏安法完成的（这里是循环伏安法（CV））。使用TU-1901双光束紫外可见（UV-VIS）分光光度计测量并记录样品，并通过X射线衍射进行相表征（XRD）以及样品的形貌和粒径通过扫描电子显微镜（SEM）进行表征。结果表明，氧化还原过程是吸附控制的，固定化的羟胺氧化酶是稳定的，在溶液中检测到的NO 2浓度在5-43 M范围内。还提到了生物传感器可以用Zno（Nps）检测改性CPE表面的羟胺。

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