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首页> 外文期刊>International Journal of Electrochemical Science >Self-assembled Diphenylalanine Peptide Fibrils with Ultra-High Aspect Ratio: A Platform for Sensitive Electrochemical H2O2 Sensor
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Self-assembled Diphenylalanine Peptide Fibrils with Ultra-High Aspect Ratio: A Platform for Sensitive Electrochemical H2O2 Sensor

机译：超高长宽比的自组装二苯丙氨酸肽原纤维：灵敏的H 2 O 2 电化学传感器平台

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摘要

Inspired from the pathogenic process of diphenylalanine (FF) self-assembly, a key structural motif informing Alzheimer’s β-amyloid peptide fibrils, a facile way for preparation of FF aromatic dipeptidefibrils and fabrication of an electrochemical biosensor were developed. Long persistence length FFfibrils with ultra-high aspect ratio over 1000 were obtained using cationic surfactant assistedevaporation induced self-assembly. The Cotton effects indicate a signature of dominant β-sheetarrangement and the thickness of the β-sheet monolayer is estimated to be 2.38 nm. The obvious blueshift of intrinsic fluorescence indicates an extended H-aggregate between the phenyl rings in a parallelmode. The FF fibrils based electrochemical biosensor, taking horseradish peroxidase as a modelenzyme, displayed an excellent electrocatalytic activity to the reduction of H2O2. The peak current wasa linear function of concentrations ranging from 7.5×10-7 to 1.4×10-5 M, with a detection limit of2.5×10-7 M. The apparent Michaelis-Menten constant was 7.2 μM. These results indicate that the FFfibrils with ultra-high aspect ratio providing a new and promising platform for construction ofelectrochemical biosensors.
机译：受二苯丙氨酸（FF）自组装的致病过程的启发，一个关键的结构基序通知了阿尔茨海默氏症的β-淀粉样肽原纤维，开发了一种制备FF芳族二肽原纤维和电化学生物传感器的简便方法。使用阳离子表面活性剂辅助蒸发诱导的自组装获得了具有超过1000的超高长径比的长持久长度FF原纤维。棉花效应表明显着的β-片层排列特征，β-片层单层的厚度估计为2.38 nm。固有荧光的明显蓝移表示在平行模式下苯环之间有扩展的H聚集。 FF原纤维为基础的电化学生物传感器，以辣根过氧化物酶为模型酶，对还原H2O2表现出优异的电催化活性。峰值电流是浓度范围为7.5×10-7至1.4×10-5 M的线性函数，检出限为2.5×10-7M。表观Michaelis-Menten常数为7.2μM。这些结果表明具有超高纵横比的FF原纤维为电化学生物传感器的构建提供了一个新的和有希望的平台。

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