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Improved Sensitivity and Selectivity Glucose Biosensor Based on PANI-GRA Nanocomposite Film Decorated with Pt Nanoparticles

机译:基于Pt纳米粒子修饰的PANI-GRA纳米复合膜的改进的灵敏度和选择性葡萄糖生物传感器

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A novel glucose biosensor was prepared by in situ electropolymerization of aniline in GRAsynthesized by using electrochemical expansion of graphite in propylene carbonate electrolyte ontoplatinum (Pt) electrode, followed by electrodeposition of platinum nanoparticles (PtNPs) on Pt/PANIGRA electrode. Glucose oxidase (GOD) was immobilized on this modified Pt electrode throughchitosan. The fabricated process and the electrochemical behaviors of resulting biosensor wereinvestigated by scanning electron microscopy (SEM) and cyclic voltammetry (CV). The developedglucose biosensor exhibited superior sensitivity of 52.67 μA mM-1 cm-2 and selectivity, which showedno response to the interference from glycine (Gly), Urea (Urea), L-phenylalanine (L-Phe), ascorbicacid (AA), tyrosine (L-Tyr) and D-galactose (D-Gal). The biosensor also showed a fast response timewithin 3 s. The biosensor was used for the detection of glucose in human blood samples withsatisfactory results compared with standard hospital laboratory analysis.
机译:通过使用碳酸亚丙酯电解质中的石墨在铂(Pt)电极上进行电化学膨胀,然后在Pt / PANIGRA电极上电沉积铂纳米粒子(PtNPs),在GRA中进行苯胺的原位电聚合,制备了一种新型葡萄糖生物传感器。葡萄糖氧化酶(GOD)通过壳聚糖固定在该修饰的Pt电极上。通过扫描电子显微镜(SEM)和循环伏安法(CV)研究了生物传感器的制备工艺和电化学行为。研发的葡萄糖生物传感器显示出52.67μAmM-1 cm-2的超强灵敏度和选择性,对甘氨酸(Gly),尿素(尿素),L-苯丙氨酸(L-Phe),抗坏血酸(AA),酪氨酸的干扰无反应(L-Tyr)和D-半乳糖(D-Gal)。该生物传感器还显示了3 s内的快速响应时间。与标准医院实验室分析相比,该生物传感器用于人血样品中葡萄糖的检测,结果令人满意。

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