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首页> 外文期刊>International Journal of Electrochemical Science >Study on the Interaction of Formononetin and Herring Sperm DNA by Electrochemical Methods and Spectroscopy
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Study on the Interaction of Formononetin and Herring Sperm DNA by Electrochemical Methods and Spectroscopy

机译：用化学方法和光谱学研究花青素与鲱鱼精子DNA的相互作用

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The interaction of formononetin (FN) with herring sperm DNA has been studied by electrochemicalmethods, UV-Vis spectroscopy and fluorescence. On a poly L-cysteine film modified electrode(PLC/GCE), FN showed a couple of well-defined voltammetric peaks in 0.1 M acetate buffer of pH5.3. As adding the herring sperm DNA into above solution, the peak current of FN decreased and nonew peaks appeared. Based on the electrochemical results, the binding constant and binding ratio4 -3 between FN and herring sperm DNA were determined to be 1.9810 M and 1:3, respectively. TheUV-Vis spectroscopy indicated that the maximum absorption of FN had obviously hyperchromiceffect and red shift after the interaction of DNA and FN. The interaction of DNA and FN can alsomake the fluorescence intensity of FN enhancement. The electrochemical and spectroscopic resultsindicated that the interaction of FN to DNA was an intercalative mode.
机译：已通过电化学方法，紫外可见光谱和荧光研究了formononetin（FN）与鲱鱼精子DNA的相互作用。在聚L-半胱氨酸膜修饰的电极（PLC / GCE）上，FN在0.1M的pH5.3乙酸盐缓冲液中显示出几个明确的伏安峰。在上述溶液中加入鲱鱼精DNA后，FN的峰值电流下降，没有新的峰值出现。基于电化学结果，FN和鲱鱼精DNA之间的结合常数和结合比4 -3分别确定为1.9810 M和1：3。 UV-Vis光谱表明，DNA与FN相互作用后，FN的最大吸收具有明显的增色作用和红移。 DNA和FN的相互作用也可以使FN的荧光强度增强。电化学和光谱结果表明，FN与DNA的相互作用是一种嵌入模式。

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