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首页> 外文期刊>International Journal of Electrochemical Science >Electrochemical Immunosensor Based on the Interactions Between Polypyrrole and Cobalt (II) Salicylaldiimine Dendrimer
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Electrochemical Immunosensor Based on the Interactions Between Polypyrrole and Cobalt (II) Salicylaldiimine Dendrimer

机译：基于聚吡咯与水杨醛二胺钴树状大分子相互作用的电化学免疫传感器

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Transglutaminase is primarily involved with activation and progression of coeliac disease through theingestion of gluten which initiates the production of antibodies against transglutaminase. This resultsin the damage of the small intestine reducing the diffusion of digested foods into the blood vessels.The diagnosis of coeliac disease can be done by the measurement of antibodies againsttransglutaminase. This work involves the development of an electrochemical immunosensor for thedetection of anti-transglutaminase antibodies. A platinum modified polypyrrole-cobalt (II)salicyladiimine metallodendrimer composite capped with transglutaminase and bovine serum albuminwas used for the determination of anti-transglutaminase antibodies. Surface roughness of 879.24 nm,76.55 nm and 364.27 nm for polypyrrole, cobalt (II) salicyladiimine metallodendrimer and theirrespective composite in conjunction with cyclic voltammetry showed that such composites provide asuitable micro-environment for monitoring antibody-antigen interactions. Electrochemical impedancespectroscopy parameters such as exchange current and heterogeneous rate constant are used to evaluate-6 2 -1 -6 2 -1 -6 2 - immunosensor where the rate constants were 2.7 x 10 cm s , 4.6 x 10 cm s and 4.16 x 10 cm s 1 for cobalt (II) salicyladiimine metallodendrimer, polypyrrole and their composite. Impedimetric-1 -5 -4 -1 immunosensor gave a limit of detection of 201 ng mL and linear range of 10 - 10 mg mL withcorrelation coefficient of 0.948.
机译：转谷氨酰胺酶主要通过谷蛋白的摄入而参与乳糜泻的活化和进展，谷蛋白的摄入会引发针对转谷氨酰胺酶的抗体的产生。这导致小肠的损伤，减少了消化食物在血管中的扩散。腹腔疾病的诊断可以通过测量转谷氨酰胺酶抗体来完成。这项工作涉及用于检测反转谷氨酰胺酶抗体的电化学免疫传感器的开发。用转谷氨酰胺酶和牛血清白蛋白封端的铂修饰的聚吡咯-钴（II）水杨醛二胺金属树枝状聚合物复合物测定抗转谷氨酰胺酶抗体。聚吡咯，钴（II）水杨二亚胺金属树枝状大分子及其各自的复合物与循环伏安法的表面粗糙度分别为879.24 nm，76.55 nm和364.27 nm，表明此类复合物为监测抗体-抗原相互作用提供了合适的微环境。电化学阻抗谱参数，例如交换电流和异质速率常数，用于评估6 2 -1 -6 2 -1 -6 -6 2-免疫传感器，其中速率常数为2.7 x 10 cm s，4.6 x 10 cm s和4.16 x 10钴（II）水杨二亚胺金属树枝状大分子，聚吡咯及其复合物的cm s 1。 Impedimetric-1 -5 -4 -1免疫传感器的检测限为201 ng mL，线性范围为10-10 mg mL，相关系数为0.948。

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