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Direct Electron Transfer Response of Superoxide Dismutase on a Graphene-Based Modified Electrode

机译:超氧化物歧化酶在石墨烯修饰电极上的直接电子转移响应

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Single stranded DNA (ssDNA)/graphene (GR) nanocomposites were prepared by a chemical route andcast on basal plane graphite (BPG) electrode to construct an ssDNA/GR modified electrode. Copper- zinc superoxide dismutase (SOD) was adsorbed tightly on the surface of the modified electrode and thedirect electron transfer of SOD was investigated using cyclic voltammetry. A couple of well-definedand quasi-reversible CV peaks of SOD can be observed and the separation of anodic and cathodic peakpotentials is 90 mV in a phosphate buffer solution (pH 7.0). The electron transfer rate constant (ks) of-1 SOD is 1.45 s . Furthermore, this biosensor exhibits high analytical performance with a wide linearrange (0.05.45 M), low detection limit (3 nM) and fast response time (1.5 s). The resultsdemonstrate that the ssDNA-graphene nanocomposite offers a biocompatible material for theconstruction of biofuel cells, bioelectronics and biosensors.
机译:通过化学路线制备单链DNA(ssDNA)/石墨烯(GR)纳米复合材料,并浇铸在基底平面石墨(BPG)电极上,以构建ssDNA / GR修饰电极。铜-锌超氧化物歧化酶(SOD)被紧密吸附在修饰电极的表面,并利用循环伏安法研究了SOD的直接电子转移。可以观察到两个SOD的定义明确且准可逆的CV峰,在磷酸盐缓冲溶液(pH 7.0)中,阳极和阴极峰电位的分离为90 mV。 -1 SOD的电子传递速率常数(ks)为1.45 s。此外,该生物传感器具有宽线性范围(0.05.45 M),低检测限(3 nM)和快速响应时间(1.5 s)的高分析性能。结果表明,ssDNA-石墨烯纳米复合材料为生物燃料电池,生物电子学和生物传感器的构建提供了生物相容性材料。

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