• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>International Journal of Electrochemical Science >Determination of Peroxidase-Like Activity of Magnetic Particles: Basic Platforms for Peroxidase Biosensors
【24h】

Determination of Peroxidase-Like Activity of Magnetic Particles: Basic Platforms for Peroxidase Biosensors

机译：磁性颗粒过氧化物酶样活性的测定：过氧化物酶生物传感器的基本平台

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Magnetic particles (MPs) are a current mean suitable for separation, biosensors construction,nanomedicine etc. They can be easily covered with a biomolecule and used for analytical purposes.Many enzymological assays have to be combined with use peroxidase. In this point of view, glucoseassay by glucose oxidase can be exampled. In the present work, we focused our attention on thedetermination whether MPs based on iron oxide have pseudo-peroxidase activity allowing use themdirectly without linking to peroxidase. Hydrogen peroxide (H2O2) as the very common substrate ofperoxidase was used for this measurement. Michaelis-Menten kinetics was performed fordemonstration of catalytic activity of MPs by measuring various concentrations of H2O2 and squarewave voltammetry (SWV), cyclic voltammetry (CV) and spectrophotometry were performed for thedetermination of pseudo-peroxidase activity. The used MPs had significant pseudo-peroxidase activityand Michaelis-Menten like behavior was observed and kinetics constants were determined. Michaelis- Menten constant (Km) was equal to 156.6 - 199.1 mmol/l (values are slightly different for the usedanalytical methods). Sensitivity of electrochemical and optical methods was also compared resulting inlimits of detection found to be 0.42 mmol/l, 0.36 mmol /l and 13.1 mmol /l for SWV, CV andspectrophotometry respectively. In a conclusion, iron oxide MPs have high pseudo-peroxidase activityresulting in opportunity to use them for the determination of hydrogen peroxide. If an oxidase appliedas a biorecognition element, there is no need to co-immobilize peroxidase. Km values were alsodetermined for HRP using spectrophotometry (25.1 mmol/l), SWV (167. mmol/l) and CV (163.2mmol/l).
机译：磁性颗粒（MPs）是目前适用于分离，生物传感器构造，纳米医学等的当前手段。它们很容易被生物分子覆盖并用于分析目的。许多酶学测定必须与过氧化物酶结合使用。从这一观点出发，可以列举通过葡萄糖氧化酶进行的葡萄糖测定。在目前的工作中，我们将注意力集中在确定基于氧化铁的MPs是否具有假过氧化物酶活性上，从而允许它们直接使用而不与过氧化物酶连接。过氧化氢（H2O2）作为过氧化物酶的最常见底物用于此测量。通过测量各种浓度的H2O2进行Michaelis-Menten动力学证明MP的催化活性，并通过方波伏安法（SWV），循环伏安法（CV）和分光光度法测定假过氧化物酶的活性。所使用的MP具有显着的假过氧化物酶活性，并观察到了Michaelis-Menten样的行为并确定了动力学常数。 Michaelis-Menten常数（Km）等于156.6-199.1 mmol / l（所用分析方法的值略有不同）。还比较了电化学方法和光学方法的灵敏度，得出SWV，CV和分光光度法的检测极限分别为0.42 mmol / l，0.36 mmol / l和13.1 mmol / l。总之，氧化铁MP具有较高的伪过氧化物酶活性，因此有机会将其用于过氧化氢的测定。如果将氧化酶用作生物识别元件，则不需要共固定过氧化物酶。还使用分光光度法（25.1 mmol / l），SWV（167. mmol / l）和CV（163.2mmol / l）测定了HRP的Km值。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号