Oriented T4 Bacteriophage Immobilization for Recognition of Escherichia coli in Capacitance Method

Danial Ashiani; Amir Homayoun Keihan; Jamal Rashidiani; Fariba Dashtestani; Khadijeh Eskandari

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Oriented T4 Bacteriophage Immobilization for Recognition of Escherichia coli in Capacitance Method

机译：电容法定向的T4噬菌体固定化识别大肠埃希菌

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One of the important aspects in biotechnology is the development of a rapid and selective approachforthe identification and quantization of Escherichia coli (E. coli) bacteria. In this research, we fabricateda novel Label-free electrochemical E. coli biosensor based on oriented immobilization of T4biotinylated bacteriophage as recognition receptor of E. coli on streptavidin immobilized on polyanilin(PANI) glassy carbon (GC) modified electrode. Labeled PANI was synthesized by chemical methodand characterized by Raman and cyclic voltammetry (CVs) and field emission scanning electronmicroscope (FESEM). The bacteriophage/PANI/GC electrodes were applied as indicator electrodes forcapacitance determination of E. coli bacteria. By fabricating this biosensor, E. coli was sensed in alinearrange and detection limit of 10-98 N/mL and 10 N/mL (N stands for number of E. coli)respectively. The proposed biosensor shows fast response time of s and good selectivity over otherbacteria. We successfully developed and implemented an E. coli biosensor based on orientedbacteriophage immobilization on PANI modified GC electrode. This biosensor is cost-effective, rapid,renewable and high selective for detection of E. coli bacteria. Also, this method can be extension todetection of other biological agents.

机译：生物技术中重要的方面之一是开发一种快速，选择性的方法来鉴定和定量大肠杆菌（E. coli）细菌。在这项研究中，我们基于固定在聚苯胺（PANI）玻碳（GC）修饰电极上的抗生蛋白链菌素上定向定位的T4生物素化噬菌体作为大肠杆菌的识别受体，制造了一种新型的无标签电化学大肠杆菌生物传感器。标记的聚苯胺通过化学方法合成，并通过拉曼和循环伏安法（CVs）和场发射扫描电子显微镜（FESEM）表征。噬菌体/ PANI / GC电极用作指示电极，用于确定大肠杆菌的电容。通过制造该生物传感器，在线性范围内检测到大肠杆菌，检测极限分别为10-98 N / mL和10 N / mL（N代表大肠杆菌数）。提出的生物传感器显示出比其他细菌更快的响应时间和良好的选择性。我们成功地开发和实现了一种基于定向噬菌体固定在PANI修饰的GC电极上的大肠杆菌生物传感器。这种生物传感器经济高效，快速，可更新且对大肠杆菌的检测具有很高的选择性。而且，该方法可以扩展到其他生物试剂的检测。

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《International Journal of Electrochemical Science》 |2016年第12期|共9页
作者
Danial Ashiani; Amir Homayoun Keihan; Jamal Rashidiani; Fariba Dashtestani; Khadijeh Eskandari;
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1. Selective and sensitive Escherichia coli Escherichia coliEscherichia coli detection based on a T4 bacteriophage‐immobilized multimode microfiber [J] . Li Yanpeng, Ma Hui, Gan Lin, Journal of Biophotonics . 2018,第9期

机译：选择性和敏感大肠杆菌大肠杆菌大肠杆菌基于T4噬菌体固定的多模超细纤维检测
2. Inside Front Cover: Selective and sensitive Escherichia coli Escherichia coliEscherichia coli detection based on a T4 bacteriophage‐immobilized multimode microfiber (J. Biophotonics 9/2018) [J] . Li Yanpeng, Ma Hui, Gan Lin, Journal of Biophotonics . 2018,第9期

机译：在前盖内：选择性和敏感大肠杆菌大肠杆菌大肠杆菌基于T4噬菌体固定化多模超细纤维检测（J.BioPhotonics 9/2018）
3. Core oligosaccharide of Escherichia coli B-the structure required for bacteriophage T4 recognition [J] . Kaszowska Marta, Niedziela Tomasz, Maciejewska Anna, Carbohydrate research . 2015,第Null期

机译：大肠杆菌B的核心寡糖-识别噬菌体T4所需的结构
4. Immobilization method of escherichia coli for microfluidic application [C] . Kim I., An T., Choi W., International Conference on Solid-State Sensors, Actuators and Microsystems . 2013

机译：用于微流控应用的大肠杆菌的固定化方法
5. Identification and Expression of the Holin Gene in Shiga-Toxigenic Escherichia coli Specific Bacteriophages and the Use of Bacteriophage Depolymerase on Stec Biofilms Formed on Food Contact Surfaces [D] . Kountoupis, Tony C. 2019

机译：Shiga-毒素大肠杆菌特异性噬菌体核心基因的鉴定和表达及在食品接触表面上形成的STEC生物膜中的噬菌体脱色酶的应用
6. Host DNA Degradation after Infection of Escherichia coli with Bacteriophage T4: Dependence of the Alternate Pathway of Degradation Which Occurs in the Absence of Both T4 Endonuclease II and Nuclear Disruption on T4 Endonuclease IV [O] . Kathleen A. Parson, D. Peter Snustad 1975

机译：大肠杆菌噬菌体T4感染后宿主DNA降解：依赖于T4核酸内切酶II和T4核酸内切酶IV都没有发生的降解的替代途径的依赖性。
7. Host DNA Degradation after Infection of Escherichia coli with Bacteriophage T4: Dependence of the Alternate Pathway of Degradation Which Occurs in the Absence of Both T4 Endonuclease II and Nuclear Disruption on T4 Endonuclease IV [O] . Kathleen A. Parson, D. Peter Snustad 1975

机译：用噬菌体T4感染大肠杆菌感染后的宿主DNA降解：在没有T4内切核酸酶II和T4内切核酸酶IV的情况下发生的降解替代途径的依赖性
8. Identification and Characterization of Mutations in 'Escherichia coli' That Selectively Influence the Growth of Hybrid Lambda Bacteriophages Carrying the Immunity Region of Bacteriophage P22 [R] . Strauch, M. A., Baumann, M., Friedman, D. I., 1986

机译：“大肠杆菌”突变的鉴定和表征，选择性地影响携带噬菌体p22免疫区的杂种λ噬菌体的生长

Oriented T4 Bacteriophage Immobilization for Recognition of Escherichia coli in Capacitance Method

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