首页> 外文期刊>International Journal of Electrochemical Science >Eelectrochemical Detection of Methyl Parathion in Fritillaria thunbergii Based on Acetylcholinesterase Immobilized Gold Nanoshpere
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Eelectrochemical Detection of Methyl Parathion in Fritillaria thunbergii Based on Acetylcholinesterase Immobilized Gold Nanoshpere

机译:基于乙酰胆碱酯酶固定化金纳米晶的贝母中对硫磷的电化学检测

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摘要

Large use of pesticide is an important issue in modern agriculture. Development of a reliable analyticalmethod for pesticide detection in essential for many fields. In this work, we proposed anelectrochemical based detection method for methyl parathion, an organophosphate pesticide, residue inthe Fritillaria thunbergii, a medicinal plant has been cultivated for several thousand years, based onthe acetylcholinesterase immobilized gold nanoshpere (Au NS) modified electrode. The combinationof acetylcholinesterase and Au NS displayed a strong synergetic effect on enhancing the detectionproperties of methyl parathion. Au NS could catalyze the oxidation process of the enzymaticallyproduced thiocholine and result a high sensitivity. After optimization, the proposed biosensor was usedfor analyzing the methyl parathion residue in freshly harvested Fritillaria thunbergii.
机译:大量使用农药是现代农业中的重要问题。在许多领域中必不可少的一种可靠的农药检测分析方法的开发。在这项工作中,我们提出了一种基于电化学的检测方法,基于乙酰胆碱酯酶固定化的金纳米晶(Au NS)修饰的电极,对贝母中的一种有机植物甲基对硫磷(一种有机磷酸酯农药)残留物进行了数千年的种植。乙酰胆碱酯酶和Au NS的结合对增强甲基对硫磷的检测性能具有很强的协同作用。 Au NS可以催化酶促产生的硫代胆碱的氧化过程,并具有很高的灵敏度。经过优化后,提出的生物传感器用于分析新鲜收获的贝母中的甲基对硫磷残留。

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