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首页> 外文期刊>International Journal of Electrochemistry >Immobilization of HRP Enzyme on Layered Double Hydroxides for Biosensor Application
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Immobilization of HRP Enzyme on Layered Double Hydroxides for Biosensor Application

机译:HRP酶在层状双氢氧化物上的固定化应用

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摘要

We present a new biosensor for hydrogen peroxide (H2O2) detection. The biosensor was based on the immobilization of horseradish peroxidase (HRP) enzyme on layered double hydroxides- (LDH-) modified gold surface. The hydrotalcite LDH (Mg2Al) was prepared by coprecipitation in constant pH and in ambient temperature. The immobilization of the peroxidase on layered hybrid materials was realized via electrostatic adsorption autoassembly process. The detection of hydrogen peroxide was successfully observed in PBS buffer with cyclic voltammetry and the chronoamperometry techniques. A limit detection of 9 μM of H2O2was obtained with a good reproducibility. We investigate the sensitivity of our developed biosensor for H2O2detection in raw milk.
机译:我们提出了一种用于过氧化氢(H2O2)检测的新型生物传感器。该生物传感器基于辣根过氧化物酶(HRP)酶在层状双氢氧化物(LDH-)修饰的金表面上的固定。通过在恒定pH和环境温度下共沉淀来制备水滑石LDH(Mg2Al)。通过静电吸附自组装过程实现了过氧化物酶在层状杂化材料上的固定化。用循环伏安法和计时电流法技术成功地在PBS缓冲液中观察到过氧化氢的检测。获得了9detectionμM的H2O2限度检测结果,重现性好。我们调查了我们开发的生物传感器对生乳中H2O2检测的敏感性。

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