• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>International Journal of Environmental Research and Public Health >Prevalence of Integrons and Insertion Sequences in ESBL-Producing E. coli Isolated from Different Sources in Navarra, Spain
【24h】

Prevalence of Integrons and Insertion Sequences in ESBL-Producing E. coli Isolated from Different Sources in Navarra, Spain

机译：从西班牙纳瓦拉的不同来源分离出的产ESBL的大肠杆菌中整合素的流行和插入序列

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Mobile genetic elements play an important role in the dissemination of antibiotic resistant bacteria among human and environmental sources. Therefore, the aim of this study was to determine the occurrence and patterns of integrons and insertion sequences of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli isolated from different sources in Navarra, northern Spain. A total of 150 isolates coming from food products, farms and feeds, aquatic environments, and humans (healthy people and hospital inpatients), were analyzed. PCRs were applied for the study of class 1, 2, and 3 integrons ( intI1 , intI2 , and intI3 ), as well as for the determination of insertion sequences (IS 26 , IS Ecp1 , IS CR1 , and IS 903 ). Results show the wide presence and dissemination of intI1 (92%), while intI3 was not detected. It is remarkable, the prevalence of intI2 among food isolates, as well as the co-existence of class 1 and class 2 (8% of isolates). The majority of isolates have two or three IS elements, with the most common being IS 26 (99.4%). The genetic pattern IS 26 –IS Ecp1 (related with the pathogen clone ST131) was present in the 22% of isolates (including human isolates). In addition, the combination IS Ecp1 –IS 26 –IS 903 –IS CR1 was detected in 11 isolates being, to our knowledge, the first study that describes this genetic complex. Due to the wide variability observed, no relationship was determined among these mobile genetic elements and β-lactam resistance. More investigations regarding the genetic composition of these elements are needed to understand the role of multiple types of integrons and insertion sequences on the dissemination of antimicrobial resistance genes among different environments.
机译：流动的遗传成分在人类和环境中的抗生素抗性细菌传播中起着重要作用。因此，本研究的目的是确定分离自西班牙北部纳瓦拉不同来源的产广谱β-内酰胺酶（ESBL）大肠杆菌的整合素的发生，模式和插入序列。共分析了150种来自食品，农场和饲料，水生环境以及人类（健康人和住院患者）的分离株。 PCR用于研究1类，2类和3类整合素（intI1，intI2和intI3），以及确定插入序列（IS 26，IS Ecp1，IS CR1和IS 903）。结果显示intI1的广泛存在和传播（92％），而未检测到intI3。值得注意的是，intI2在食物分离株中的流行，以及1类和2类（8％的分离株）并存。大多数分离株具有两个或三个IS元素，最常见的是IS 26（99.4％）。 22％的分离株（包括人类分离株）中存在遗传模式IS 26 –IS Ecp1（与病原体克隆ST131相关）。此外，据我们所知，在11个分离株中检测到IS Ecp1 –IS 26 –IS 903 –IS CR1的组合，这是第一个描述这种遗传复合物的研究。由于观察到的广泛变异性，这些流动的遗传元件和β-内酰胺抗性之间没有确定的关系。需要更多有关这些元素的遗传组成的研究，以了解多种类型的整合素和插入序列在不同环境中抗菌素耐药基因的传播中的作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号