...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>International Journal of Environmental Research and Public Health >In Vitro and In Vivo Control of Secondary Bacterial Infection Caused by Leishmania major
【24h】

In Vitro and In Vivo Control of Secondary Bacterial Infection Caused by Leishmania major

机译：利什曼原虫引起的继发细菌感染的体外和体内控制

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Bacterial infections of cutaneous leishmaniasis cause skin ulcers on mice, resulting in increased tissue deterioration, and these infections can be controlled with liquid allicin. To isolate and identify the incidences of real secondary bacterial infections in mice, we performed the current study by injecting mice ( n = 50) with Leishmania major. L. major infections were initiated by an intramuscular injection of 0.1 mL Roswell Park Memorial Institute (RPMI 1640 media/mouse (10 7 promastigote/mL)). Scarring appeared 2–6 weeks after injection, and the bacteria were isolated from the skin ulcer tissues. Allicin (50 μL/mL) and ciprofloxacin (5 μg; Cip 5) were used for controlling L. major and bacteria. One hundred samples from skin ulcers of mice were examined, and 200 bacterial colonies were isolated. Forty-eight different genera and species were obtained and identified by Gram staining and physiological and biochemical characterization using identification kits. All samples were positive for secondary bacterial infections. Of the isolates, 79.16% were identified as Gram-negative bacteria, and 28.84% were identified as Gram-positive bacteria; only one yeast species was found. Interestingly, pure allicin liquid at a concentration 50 μL/mL exhibited antibacterial activity against a wide range of Gram-negative and some Gram-positive bacteria, in addition to yeast, and was 71.43% effective. Antimicrobial resistance patterns of all genera and species were determined using 15 different antibiotics. Allicin (50 μL/mL) and Cip 5 were the most effective against L. major and 92.30% of isolated bacteria. Stenotrophomonas maltophilia was the most resistant bacterium to the tested antibiotics with a survival rate of 73.33%, and it exhibited resistance to allicin.
机译：皮肤利什曼病的细菌感染在小鼠上引起皮肤溃疡，导致组织恶化加剧，并且可以用液体大蒜素来控制这些感染。为了分离和鉴定小鼠中真正的继发细菌感染的发生率，我们通过向小鼠（n = 50）注射利​​什曼原虫进行了本研究。 L.重大感染通过肌肉内注射0.1 mL罗斯威尔公园纪念研究所（RPMI 1640培养基/小鼠（10 7前鞭毛体/ mL））引发。注射后2-6周出现疤痕，细菌从皮肤溃疡组织中分离出来。大蒜素（50μL/ mL）和环丙沙星（5μg; Cip 5）用于控制大肠埃希菌和细菌。检查了来自小鼠皮肤溃疡的一百个样品，并分离了200个细菌菌落。获得了48个不同的属和种，并通过革兰氏染色和使用鉴定试剂盒的生理生化特征进行鉴定。所有样品的继发细菌感染均为阳性。在分离物中，有79.16％被鉴定为革兰氏阴性菌，有28.84％被鉴定为革兰氏阳性菌。仅发现一种酵母。有趣的是，浓度为50μL/ mL的纯大蒜素液体除对酵母菌外，还对多种革兰氏阴性菌和某些革兰氏阳性菌表现出抗菌活性，有效率为71.43％。使用15种不同的抗生素确定所有属和种的抗药性模式。大蒜素（50μL/ mL）和Cip 5对大肠埃希菌和92.30％的分离细菌最有效。嗜麦芽窄食单胞菌是对测试抗生素最有抵抗力的细菌，存活率为73.33％，并且对大蒜素表现出抵抗力。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号