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首页> 外文期刊>International Journal of Environmental Research and Public Health >Mitogenic and Cytotoxic Effects of Pentachlorophenol to AML 12 Mouse Hepatocytes
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Mitogenic and Cytotoxic Effects of Pentachlorophenol to AML 12 Mouse Hepatocytes

机译：五氯苯酚对AML 12小鼠肝细胞的致突变和细胞毒性作用

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Pentachlorophenol (PCP), an organochlorine fungicide, is extensively used in the United States for the protection of wood products. Moreover, widespread agricultural, domestic, and industrial applications have caused PCP-contaminants to enter the food chain from the environment. There is accumulating evidence indicating that PCP is highly toxic to humans, and causes injury to major organs including the lung, liver, kidneys, heart, and brain. While PCP has been shown to induce systemic toxicity and carcinogenesis in several experimental studies, the literature is scarce regarding its toxic mechanisms of action. Recent investigations in our laboratory have shown that PCP exerts both cytotoxic and mitogenic effects in human liver carcinoma (HepG2) cells [1], and in primary culture of catfish hepatocytes [2]. In the present study, we hypothesized that PCP exposure will trigger similar cytotoxic and mitogenic responses in AML 12 Mouse hepatocytes. To test this hypothesis, we performed the MTT assay for cell viability in PCP-treated and control cells. Data obtained from this experiment indicated a biphasic response with respect to PCP toxicity; showing a hormosis effect characterized by mitogenicity at lower levels of exposure, and cytotoxicity at higher doses. Upon 48 hrs of exposure, PCP chemical doses required to cause 50% reduction in the viability (LC50) of AML 12 mouse hepatocytes was computed to be 16.0 + 2.0 μg/mL. These results indicate that, although the sensitivity to PCP toxicity varies from one cell line to another, its toxic mechanisms are similar across cell lines.
机译：五氯苯酚（PCP）是一种有机氯杀真菌剂，在美国广泛用于保护木制品。此外，广泛的农业，家庭和工业应用已导致五氯苯酚污染物从环境进入食物链。越来越多的证据表明，五氯苯酚对人有剧毒，并会损害包括肺，肝，肾，心脏和大脑在内的主要器官。尽管在一些实验研究中已证明五氯苯酚可诱导全身毒性和致癌作用，但有关其毒性作用机理的文献很少。我们实验室的最新研究表明，五氯苯酚对人肝癌（HepG2）细胞[1]和，鱼肝细胞的原代培养物[2]均具有细胞毒性和促有丝分裂作用。在本研究中，我们假设PCP暴露将在AML 12小鼠肝细胞中引发类似的细胞毒性和促有丝分裂反应。为了检验该假设，我们对PCP处理和对照细胞中的细胞活力进行了MTT分析。从该实验获得的数据表明，对PCP毒性具有双相反应。表现出以低剂量的有丝分裂性和高剂量的细胞毒性为特征的激素效应。暴露48小时后，导致AML 12小鼠肝细胞活力（LC50）降低50％所需的PCP化学剂量计算为16.0 + 2.0μg/ mL。这些结果表明，尽管对PCP毒性的敏感性从一种细胞系到另一种细胞系都不同，但其毒性机制在整个细胞系中是相似的。

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