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首页> 外文期刊>International Journal of Environmental Research and Public Health >The Kinetic Signature of Toxicity of Four Heavy Metals and Their Mixtures on MCF7 Breast Cancer Cell Line?
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The Kinetic Signature of Toxicity of Four Heavy Metals and Their Mixtures on MCF7 Breast Cancer Cell Line?

机译：四种重金属及其混合物对MCF7乳腺癌细胞系毒性的动力学特征？

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This study evaluated the kinetic signature of toxicity of four heavy metals known to cause severe health and environmental issues—cadmium (Cd), mercury (Hg) lead (Pb) arsenic (As)—and the mixture of all four metals (Mix) on MCF7 cancer cells, in the presence and absence of the antioxidant glutathione (GSH). The study was carried out using real time cell electronic sensing (RT-CES). RT-CES monitors in real time the electrical impedance changes at the electrode/culture medium interface due to the number of adhered cells, which is used as an index of cell viability. Cells were seeded for 24 h before exposure to the metals and their mixtures. The results showed that in the presence and absence of cellular glutathione, arsenic was the most cytotoxic of all five treatments, inducing cell death after 5 h of exposure. Lead was the least cytotoxic in both scenarios. In the presence of cellular GSH, the cytotoxic trend was As > Cd > MIX > Hg > Pb, while in the absence of GSH, the cytotoxic trend was As > Hg > MIX > Cd > Pb. The findings from this study indicate the significance of glutathione-mediated toxicity of the metals examined—particularly for mercury—and may be clinically relevant for disorders such as autism spectrum disorder where decreased glutathione-based detoxification capacity is associated with increased mercury intoxication.
机译：这项研究评估了已知会导致严重健康和环境问题的四种重金属的毒性的动力学特征-镉（Cd），汞（Hg）铅（Pb）砷（As）-以及四种四种金属的混合物（Mix）在存在和不存在抗氧化剂谷胱甘肽（GSH）的情况下，MCF7癌细胞。该研究使用实时细胞电子传感（RT-CES）进行。 RT-CES实时监控电极/培养基界面上由于粘附的细胞数量而引起的电阻抗变化，该电阻率被用作细胞存活率的指标。在暴露于金属及其混合物之前，将细胞接种24小时。结果表明，在存在和不存在细胞谷胱甘肽的情况下，砷是所有五种治疗方法中最具细胞毒性的，导致暴露5 h后细胞死亡。在这两种情况下，铅的细胞毒性最小。在存在细胞GSH的情况下，细胞毒性趋势为As> Cd> MIX> Hg> Pb，而在没有GSH的情况下，细胞毒性趋势为As> Hg> MIX> Cd> Pb。这项研究的结果表明，谷胱甘肽介导的被测金属的毒性具有重要意义，尤其是对于汞，并且在临床上与自闭症谱系障碍等疾病相关，其中基于谷胱甘肽的解毒能力降低与汞中毒增加有关。

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