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首页> 外文期刊>International Journal of Environmental Research and Public Health >Evaluation of the Relationship between the Adenosine Triphosphate (ATP) Bioluminescence Assay and the Presence of Bacillus anthracis Spores and Vegetative Cells
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Evaluation of the Relationship between the Adenosine Triphosphate (ATP) Bioluminescence Assay and the Presence of Bacillus anthracis Spores and Vegetative Cells

机译：三磷酸腺苷（ATP）生物发光测定与炭疽芽孢杆菌孢子和营养细胞的存在之间的关系的评价

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摘要

The Adenosine triphosphate (ATP) bioluminescence assay was utilized in laboratory evaluations to determine the presence and concentration of vegetative and spore forms of Bacillus anthracis Sterne 34F2. Methods: Seventeen surfaces from the healthcare environment were selected for evaluation. Surfaces were inoculated with 50 μL of organism suspensions at three concentrations of 104, 106, 108 colony forming units per surface (CFU/surface) of B. anthracis. Culture-based methods and ATP based methods were utilized to determine concentrations. Results: When all concentrations were evaluated together, a positive correlation between log-adjusted CFU and Relative Light Units (RLU) for endospores and vegetative cells was established. When concentrations were evaluated separately, a significant correlation was not demonstrated. Conclusions: This study demonstrated a positive correlation for ATP and culture-based methods for the vegetative cells of B. anthracis. When evaluating the endospores and combining both metabolic states, the ATP measurements and CFU recovered did not correspond to the initial concentrations on the evaluated surfaces. The results of our study show that the low ATP signal which does not correlate well to the CFU results would not make the ATP measuring devises effective in confirming contamination residual from a bioterrorist event.
机译：三磷酸腺苷（ATP）生物发光法用于实验室评估，以确定炭疽芽孢杆菌Sterne 34F2的营养和孢子形式的存在和浓度。方法：从医疗环境中选择17个表面进行评估。用炭疽芽孢杆菌每个表面（CFU /表面）三个浓度的104、106、108菌落形成单位的浓度分别以50μL的生物悬浮液接种表面。利用基于培养物的方法和基于ATP的方法确定浓度。结果：当所有浓度一起评估时，对数调整后的CFU与内生孢子和营养细胞的相对光单位（RLU）之间建立正相关。当分别评估浓度时，没有显示出显着的相关性。结论：这项研究证明了炭疽芽孢杆菌营养细胞的ATP和基于培养的方法呈正相关。当评估孢子并结合两种代谢状态时，ATP测量值和回收的CFU与评估表面上的初始浓度不符。我们的研究结果表明，与CFU结果不完全相关的低ATP信号不会使ATP测量装置有效地确认生物恐怖事件的残留污染。

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