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首页> 外文期刊>International Journal of Environmental Research and Public Health >Prevalence of Methicillin-Resistant Staphylococcus aureus and Other Staphylococcus Species in Raw Meat Samples Intended for Human Consumption in Benin City, Nigeria: Implications for Public Health
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Prevalence of Methicillin-Resistant Staphylococcus aureus and Other Staphylococcus Species in Raw Meat Samples Intended for Human Consumption in Benin City, Nigeria: Implications for Public Health

机译：尼日利亚贝宁市用于人类消费的生肉样品中耐甲氧西林金黄色葡萄球菌和其他葡萄球菌的流行：对公共卫生的影响

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The present study was designed to characterize methicillin-resistant staphylococci from raw meat. A total of 126 meat samples were obtained from open markets between February and April, 2015. Antimicrobial susceptibility testing was carried out using the disc diffusion method. Molecular profiling was conducted using 16S rRNA, mec A, nuc , and PVL gene signatures were detected by polymerase chain reaction assay. Fifty isolates of methicillin-resistant Staphylococcus spp. were detected in 26 (52%) pork, 14 (28%) beef and 10 (20%) chicken samples. The staphylococcal isolates were identified through partial 16S ribosomal ribonucleic acid (16S rRNA) nucleotide sequencing, and BLAST analysis of the gene sequence revealed 98%–100% staphylococcal similarity. All isolates from beef and chicken samples amplified the mec A gene, while 100% of the MRSA isolates amplified the PVL gene. The multidrug resistance profile (resistant to ≥1 antimicrobial agent in ≥3 classes of antimicrobial agents) of the staphylococcal isolates showed that 7 isolates were resistant to methicillin, penicillin, clindamycin, chloramphenicol, trimethoprim-sulfamethoxazole, kanamycin, amoxicillin, cloxacillin, erythromycin, vancomycin, and gentamycin. There was a significant regression effect from the multidrug-resistant profile on the number of isolates ( p < 0.05) suggesting a consequence of the dissemination of resistant strains within bacterial populations. The findings of the present study indicate that raw meats in the Benin metropolis were possibly contaminated with pathogenic and multi-drug resistant staphylococci strains and therefore could constitute a risk to public health communities.
机译：本研究旨在表征生肉中耐甲氧西林的葡萄球菌。 2015年2月至4月之间，总共从公开市场上获得了126个肉样品。使用圆盘扩散法进行了药敏试验。使用16S rRNA进行分子谱分析，mec A，nuc和通过聚合酶链反应测定法检测PVL基因标记。五十株耐甲氧西林葡萄球菌的分离株。在26份（52％）猪肉，14份（28％）牛肉和10份（20％）鸡肉样品中检测到了豆蔻。通过部分16S核糖体核糖核酸（16S rRNA）核苷酸测序鉴定出葡萄球菌分离物，基因序列的BLAST分析显示出98％-100％的葡萄球菌相似性。来自牛肉和鸡肉样品的所有分离株均扩增了mec A基因，而100％的MRSA分离株均扩增了PVL基因。葡萄球菌分离株的多药耐药性（对≥3种抗菌剂中的≥1种抗菌剂有耐药性）显示，有7种分离株对甲氧西林，青霉素，克林霉素，氯霉素，甲氧苄氨嘧啶-磺胺甲恶唑，卡那霉素，阿莫西林，氯氧西林，氯氧西林万古霉素和庆大霉素。多重耐药性谱对分离株的数量具有显着的回归作用（p <0.05），表明耐药菌在细菌种群中的传播。本研究的结果表明，贝宁大都市的生肉可能被致病性和多药耐药性葡萄球菌菌株污染，因此可能对公共卫生社区构成风险。

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