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首页> 外文期刊>International Journal of Environmental Research and Public Health >Bioaerosols from a Food Waste Composting Plant Affect Human Airway Epithelial Cell Remodeling Genes
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Bioaerosols from a Food Waste Composting Plant Affect Human Airway Epithelial Cell Remodeling Genes

机译：食物垃圾堆肥厂中的生物气溶胶影响人类气道上皮细胞重塑基因。

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The composting procedure in food waste plants generates airborne bioaerosols that have the potential to damage human airway epithelial cells. Persistent inflammation and repair responses induce airway remodeling and damage to the respiratory system. This study elucidated the expression changes of airway remodeling genes in human lung mucoepidermoid NCI-H292 cells exposed to bioaerosols from a composting plant. Different types of microorganisms were detectable in the composting plant, using the agar culture method. Real-time polymerase chain reaction was used to quantify the level of Aspergillus fumigatus and the profile of remodeling genes. The real-time PCR results indicated that the amount of A. fumigatus in the composting hall was less than 102 conidia. The endotoxins in the field bioaerosols were determined using a limulus amebocyte lysate test. The endotoxin levels depended on the type of particulate matter (PM), with coarse particles (2.5–10 μm) having higher endotoxin levels than did fine particles (0.5–2.5 μm). After exposure to the conditioned medium of field bioaerosol samples, NCI-H292 cells showed increased pro-inflammatory interleukin (IL)-6 release and activated epidermal growth factor receptor (EGFR), transforming growth factor (TGF)-β1 and cyclin-dependent kinase inhibitor 1 (p21WAF1/CIP1) gene expression, but not of matrix metallopeptidase (MMP)-9. Airborne endotoxin levels were higher inside the composting hall than they were in other areas, and they were associated with PM. This suggested that airborne bioaerosols in the composting plant contained endotoxins and microorganisms besides A. fumigatus that cause the inflammatory cytokine secretion and augment the expression of remodeling genes in NCI-H292 cells. It is thus necessary to monitor potentially hazardous materials from bioaerosols in food composting plants, which could affect the health of workers.
机译：食物垃圾处理厂中的堆肥程序会产生空气传播的生物气溶胶，这些生物气溶胶可能会破坏人的呼吸道上皮细胞。持续的炎症和修复反应会引起气道重塑并损害呼吸系统。这项研究阐明了暴露于堆肥植物生物气溶胶的人肺粘液表皮样NCI-H292细胞中气道重塑基因的表达变化。使用琼脂培养法可在堆肥植物中检测到不同类型的微生物。实时聚合酶链反应用于定量烟曲霉的水平和重塑基因的概况。实时PCR结果表明，堆肥大厅中烟曲霉的数量少于102个分生孢子。田间生物气溶胶中的内毒素使用变形细胞溶解物测试进行测定。内毒素水平取决于颗粒物（PM）的类型，粗颗粒（2.5–10μm）的内毒素水平高于细颗粒（0.5–2.5μm）。暴露于现场生物气溶胶样品的条件培养基后，NCI-H292细胞显示出促炎性白介素（IL）-6释放增加和活化的表皮生长因子受体（EGFR），转化生长因子（TGF）-β1和细胞周期蛋白依赖性激酶抑制剂1（p21WAF1 / CIP1）基因表达，但不表达基质金属肽酶（MMP）-9。堆肥大厅内的空气传播的内毒素水平高于其他地区，并且与PM有关。这表明，堆肥植物中的空气传播生物气溶胶除烟曲霉外还含有内毒素和微生物，这些细菌会引起炎症性细胞因子分泌并增加NCI-H292细胞中重塑基因的表达。因此，有必要监测食物堆肥厂中来自生物气溶胶的潜在有害物质，这些物质可能会影响工人的健康。

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