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首页> 外文期刊>International Journal of Environmental Research and Public Health >Abundance of Pathogenic Escherichia coli Virulence-Associated Genes in Well and Borehole Water Used for Domestic Purposes in a Peri-Urban Community of South Africa
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Abundance of Pathogenic Escherichia coli Virulence-Associated Genes in Well and Borehole Water Used for Domestic Purposes in a Peri-Urban Community of South Africa

机译：南非周边城市社区用于家庭目的的井水和井水中致病性大肠杆菌毒力相关基因的丰富性

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In the absence of pipe-borne water, many people in Africa, especially in rural communities, depend on alternative water sources such as wells, boreholes and rivers for household and personal hygiene. Poor maintenance and nearby pit latrines, however, lead to microbial pollution of these sources. We evaluated the abundance of Escherichia coli and the prevalence of pathogenic E. coli virulence genes in water from wells, boreholes and a river in a South African peri-urban community. Monthly samples were collected between August 2015 and November 2016. In all, 144 water samples were analysed for E. coli using the Colilert 18 system. Virulence genes ( eagg , eaeA , stx1 , stx2 , flichH7 , ST , ipaH , ibeA ) were investigated using real-time polymerase chain reaction. Mean E. coli counts ranged between 0 and 443.1 Most Probable Number (MPN)/100 mL of water sample. Overall, 99.3% of samples were positive for at least one virulence gene studied, with flicH7 being the most detected gene (81/140; 57.6%) and the stx2 gene the least detected gene (8/140; 5.7%). Both intestinal and extraintestinal pathogenic E. coli genes were detected. The detection of virulence genes in these water sources suggests the presence of potentially pathogenic E. coli strains and is a public health concern.
机译：在没有管道供水的情况下，非洲的许多人，尤其是农村社区的人们，依赖水井，井眼和河流等替代水源来维持家庭和个人卫生。但是，维护不善和附近的坑厕导致这些源的微生物污染。我们评估了南非郊区社区水井，井眼和河流中水的大肠杆菌含量和致病性大肠杆菌毒力基因的普遍性。在2015年8月至2016年11月之间每月收集样品。使用Colilert 18系统对144个水样中的大肠杆菌进行了分析。使用实时聚合酶链反应研究了毒力基因（eagg，eaeA，stx1，stx2，flichH7，ST，ipaH，ibeA）。大肠杆菌的平均计数范围为0到443.1最可能数（MPN）/ 100 mL水样品。总体而言，至少有一个研究的毒力基因样本中99.3％呈阳性，其中flicH7是检测到最多的基因（81/140; 57.6％），而stx2基因是检测到最少的基因（8/140; 5.7％）。肠道和肠道外的致病性大肠杆菌基因都被检测到。这些水源中毒力基因的检测表明存在潜在致病性的大肠杆菌菌株，这是公共卫生问题。

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