...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>International Journal of Environmental Research and Public Health >Occurrence of Virulence Genes Associated with Diarrheagenic Escherichia coli Isolated from Raw Cow’s Milk from Two Commercial Dairy Farms in the Eastern Cape Province, South Africa
【24h】

Occurrence of Virulence Genes Associated with Diarrheagenic Escherichia coli Isolated from Raw Cow’s Milk from Two Commercial Dairy Farms in the Eastern Cape Province, South Africa

机译：从南非东开普省两个商业奶牛场的生牛奶中分离到的致泻性大肠杆菌相关毒力基因的发生

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Escherichia coli remains a public health concern worldwide as an organism that causes diarrhea and its reservoir in raw milk may play an important role in the survival and transport of pathogenic strains. Diarrheagenic E. coli strains are diverse food-borne pathogens and causes diarrhea with varying virulence in humans. We investigated the prevalence of pathogenic E. coli in raw milk from two commercial dairy farms. Four hundred raw milk samples, 200 from each dairy farm, were screened for the presence of fliCH7, eagR, ial, eagg, lt, and papC genes. In dairy farm A, 100 E. coli were identified based on culture, oxidase and Gram staining, while 88 isolates from dairy farm B were identified in the same manner. Gene detection showed fliCH7 27 (54%) to be the highest gene detected from farm A and lt 2 (4%) to be the lowest. The highest gene detected in dairy farm B was fliCH7 16 (43.2%) and papC 1 (2.7%) was the least. The amplification of pathogenic genes associated with diarrheagenic E. coli from cows’ raw milk demonstrates that potentially virulent E. coli strains are widely distributed in raw milk and may be a cause of concern for human health.
机译：大肠埃希氏菌是引起腹泻的一种有机体，在全球范围内仍然是公共卫生问题，其在生乳中的储藏可能在致病菌株的存活和运输中起重要作用。引起腹泻的大肠杆菌菌株是多种食源性病原体，会在人类中引起不同毒力的腹泻。我们调查了来自两个商业奶牛场的原奶中致病性大肠杆菌的患病率。从fliCH7，eagR，ial，eagg，lt和papC基因的存在中筛选了400个原奶样品（每个奶牛场200个）。在奶牛场A中，根据培养，氧化酶和革兰氏染色鉴定了100株大肠杆菌，同时以相同方式鉴定了从奶牛场B中分离出的88株大肠杆菌。基因检测显示，fliCH7 27（54％）是从农场A检测到的最高基因，lt 2（4％）是最低的。在奶牛场B中检测到的最高基因是fliCH7 16（43.2％），而papC 1（2.7％）最少。牛原奶中与腹泻性大肠杆菌相关的致病基因的扩增表明，潜在的强毒大肠杆菌菌株广泛分布在原奶中，可能引起人类健康。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号