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首页> 外文期刊>International Journal of Environmental Research and Public Health >Testis-Specific Lactate Dehydrogenase (LDH-C4) in Skeletal Muscle Enhances a Pika’s Sprint-Running Capacity in Hypoxic Environment
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Testis-Specific Lactate Dehydrogenase (LDH-C4) in Skeletal Muscle Enhances a Pika’s Sprint-Running Capacity in Hypoxic Environment

机译：骨骼肌中睾丸特异性乳酸脱氢酶（LDH-C 4 ）增强了低氧环境下皮卡的短跑能力

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LDH-C4 is a lactate dehydrogenase that catalyzes the conversion of pyruvate to lactate. In mammals, ldh-c was originally thought to be expressed only in testis and spermatozoa. Plateau pika (Ochotona curzoniae), which belongs to the genus Ochotona of the Ochotonidea family, is a hypoxia tolerant mammal living 3000–5000 m above sea level on the Qinghai-Tibet Plateau, an environment which is strongly hypoxic. Ldh-c is expressed not only in testis and sperm but also in somatic tissues of plateau pika. In this study, the effects of N-propyl oxamate and N-isopropyl oxamate on LDH isozyme kinetics were compared to screens for a selective inhibitor of LDH-C4. To reveal the role and physiological mechanism of LDH-C4 in skeletal muscle of plateau pika, we investigated the effect of N-isopropyl oxamate on the pika exercise tolerance as well as the physiological mechanism. Our results show that Ki of N-propyl oxamate and N-isopropyl oxamate for LDH-A4, LDH-B4, and LDH-C4 were 0.094 mmol/L and 0.462 mmol/L, 0.119 mmol/L and 0.248 mmol/L, and 0.015 mmol/L and 0.013 mmol/L, respectively. N-isopropyl oxamate is a powerful selective inhibitor of plateau pika LDH-C4. In our exercise tolerance experiment, groups treated with inhibitors had significantly lower swimming times than the uninhibited control group. The inhibition rates of LDH, LD, and ATP were 37.12%, 66.27%, and 32.42%, respectively. Our results suggested that ldh-c is expressed in the skeletal muscle of plateau pika, and at least 32.42% of ATP in the skeletal muscle is catalyzed by LDH-C4 by anaerobic glycolysis. This suggests that pika has reduced dependence on oxygen and enhanced adaptation to hypoxic environment due to increased anaerobic glycolysis by LDH-C4 in skeletal muscle. LDH-C4 in plateau pika plays the crucial role in anaerobic glycolysis and generates ATP rapidly since this is the role of LDH-A4 in most species on plain land, which provide evidence that the native humans and animals in Qinghai-Tibet plateau can adapt to the hypoxia environment.
机译：LDH-C 4 是一种乳酸脱氢酶，可催化丙酮酸转化为乳酸。在哺乳动物中，ldh-c最初被认为仅在睾丸和精子中表达。高原鼠兔（Ochotona curzoniae）属于Ochotonidea家族的Ochotona属，是一种耐缺氧的哺乳动物，生活在高度缺氧的青藏高原上，海拔3000-5000 m。 Ldh-c不仅在睾丸和精子中表达，而且在高原鼠兔的体细胞组织中也表达。在这项研究中，将草酸N-丙酯和草酸N-异丙基酯对LDH同工酶动力学的影响与筛选LDH-C 4 的选择性抑制剂进行了比较。为了揭示LDH-C 4 在高原鼠兔骨骼肌中的作用及其生理机制，我们研究了草酸N-异丙基酯对鼠兔运动耐性的影响及其生理机制。我们的结果表明，草酸N-丙酯和草酸N-异丙基酯对LDH-A 4 ，LDH-B 4 和LDH-C 4 4 的有效选择性抑制剂。在我们的运动耐量实验中，用抑制剂治疗的组的游泳时间明显少于未抑制的对照组。 LDH，LD和ATP的抑制率分别为37.12％，66.27％和32.42％。我们的研究结果表明，ldh-c在高原鼠兔的骨骼肌中表达，而厌氧糖酵解通过LDH-C 4 催化了骨骼肌中至少32.42％的ATP。这表明由于LDH-C 4 对骨骼肌的厌氧糖酵解作用增加，皮卡对氧气的依赖性降低，对缺氧环境的适应性增强。高原鼠兔中的LDH-C 4 在厌氧糖酵解中起关键作用，并迅速产生ATP，因为这是平原地区大多数物种中LDH-A 4 的作用。提供证据表明青藏高原的本地人和动物可以适应低氧环境。

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