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首页> 外文期刊>International Journal of Environmental Research and Public Health >Intrahippocampal Infusion of Crotamine Isolated from Crotalus durissus terrificus Alters Plasma and Brain Biochemical Parameters †
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Intrahippocampal Infusion of Crotamine Isolated from Crotalus durissus terrificus Alters Plasma and Brain Biochemical Parameters †

机译：从海螯虾中分离出的可乐明海马内输注改变血浆和脑生化参数†

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摘要

Crotamine is one of the main constituents of the venom of the South American rattlesnake Crotalus durissus terrificus. Here we sought to investigate the inflammatory and toxicological effects induced by the intrahippocampal administration of crotamine isolated from Crotalus whole venom. Adult rats received an intrahippocampal infusion of crotamine or vehicle and were euthanized 24 h or 21 days after infusion. Plasma and brain tissue were collected for biochemical analysis. Complete blood count, creatinine, urea, glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT), creatine-kinase (CK), creatine kinase-muscle B (CK-MB) and oxidative parameters (assessed by DNA damage and micronucleus frequency in leukocytes, lipid peroxidation and protein carbonyls in plasma and brain) were quantified. Unpaired and paired t-tests were used for comparisons between saline and crotamine groups, and within groups (24 h vs. 21 days), respectively. After 24 h crotamine infusion promoted an increase of urea, GOT, GPT, CK, and platelets values (p ≤ 0.01), while red blood cells, hematocrit and leukocytes values decreased (p ≤ 0.01). Additionally, 21 days after infusion crotamine group showed increased creatinine, leukocytes, TBARS (plasma and brain), carbonyl (plasma and brain) and micronucleus compared to the saline-group (p ≤ 0.01). Our findings show that crotamine infusion alter hematological parameters and cardiac markers, as well as oxidative parameters, not only in the brain, but also in the blood, indicating a systemic pro-inflammatory and toxicological activity. A further scientific attempt in terms of preserving the beneficial activity over toxicity is required.
机译：巴豆胺是南美响尾蛇响尾蛇的毒液的主要成分之一。在这里，我们试图研究由海马内从克塔卢斯全毒中分离出来的巴豆胺引起的炎症和毒理作用。成年大鼠接受海马内巴豆胺或赋形剂输注，并在输注后24小时或21天实施安乐死。收集血浆和脑组织用于生化分析。全血细胞计数，肌酐，尿素，谷草草酰乙酸转氨酶（GOT），谷氨酸丙酮酸转氨酶（GPT），肌酸激酶（CK），肌酸激酶-肌肉B（CK-MB）和氧化参数（通过DNA损伤和微核频率评估）在白细胞中，对血浆和大脑中的脂质过氧化和蛋白质羰基化合物进行了定量。未配对和配对t检验分别用于盐水组和巴豆胺组之间以及组内（24小时对21天）的比较。 24小时后，巴豆胺输注促进尿素，GOT，GPT，CK和血小板值的增加（p≤0.01），而红细胞，血细胞比容和白细胞值下降（p≤0.01）。此外，与盐水组相比，克罗他敏组在输注后21天显示肌酐，白细胞，TBARS（血浆和脑），羰基（血浆和脑）和微核增加（p≤0.01）。我们的发现表明，巴豆胺的输注不仅会改变大脑，而且还会改变血液中的血液学参数和心脏标志物以及氧化参数，表明其具有全身性促炎和毒理学活性。在保持有益的活性而不是毒性方面需要进一步的科学尝试。

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