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首页> 外文期刊>International Journal of Agriculture and Biology >Genetic Diversity of Primary Core Kernel-apricot Germplasms using ISSR Markers
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Genetic Diversity of Primary Core Kernel-apricot Germplasms using ISSR Markers

机译：利用ISSR标记对核心核心杏杏种质的遗传多样性

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The aim of this study was to screen the germplasm for desirable traits such as high yield, better kernel quality and strong stress resistance, using collected germplasm resources to establish a core breeding population. Using inter-simple sequence repeat (ISSR) molecular markers, we analyzed 47 initially selected germplasm samples of kernel-apricot with high yield, good quality, and resistance to late spring frost for their nucleotide sequence polymorphism. Sixteen ISSR primers with high polymorphism, clear bands, and good resolution were selected from 100 primers. These primers amplified 126 loci from the test samples, of which 85.7% were polymorphic. The amplified bands were characterized by Shannon index (I) of 0.4167 and Nei's diversity index (He) of 0.267; the effective number of alleles (Ne) was 1.4286, genetic identity ranged from 0.4048 to 1.0000, and genetic distance was between 0.0008 and 0.9045. At the genetic similarity coefficient of 0.734, the 47 accessions of kernel-apricot germplasm were divided into three groups. In contrast, principal component analysis resolved these accessions into only two groups, indicating the differences between the two statistical methods in their assessment of the relationships among individual cultivars. The stepwise sampling revealed that the highest effective number of alleles was obtained after four rounds of sampling, whereas Nei's gene diversity and Shannon information indices reached maximum values after five rounds of sampling, although the percentage of polymorphic loci significantly decreased. The number of germplasm resources also dropped to about 27.7% of the total number before sampling, which was no longer representative. In summary, the 16 ISSR polymorphic primers screened and selected in this study, combined with four consecutive stepwise sampling events at minimum distance based on cluster analysis, screened 19 samples whose ratio reached to 40.4% of the whole core germplasm. The high Ne, He, and I of the core germplasms indicated that this strategy produces results that can be used for further kernel-apricot germplasm improvement.
机译：这项研究的目的是利用收集的种质资源建立核心育种群体，筛选出具有高产，更好的籽粒质量和较强的抗逆性等理想性状的种质。使用简单序列间重复（ISSR）分子标记，我们分析了47个最初选择的核仁杏种质样品，它们的核苷酸序列多态性高产，优质，对春末霜冻具有抗性。从100个引物中选择了16个具有高多态性，清晰条带和良好分辨率的ISSR引物。这些引物从测试样品中扩增了126个基因座，其中85.7％是多态性的。扩增的条带的特征在于香农指数（I）为0.4167，尼氏多样性指数（He）为0.267;等位基因（Ne）的有效数量为1.4286，遗传同一性在0.4048至1.0000之间，遗传距离在0.0008至0.9045之间。在0.734的遗传相似系数下，将47个核仁杏种质分为三组。相比之下，主成分分析将这些种仅分为两组，表明两种统计方法在评估各个品种之间的关系时存在差异。逐步采样显示，经过四轮采样后，等位基因的有效数量最高，而五轮采样后，Nei的基因多样性和Shannon信息指数达到最大值，尽管多态性位点的百分比明显降低。种质资源的数量也下降到取样前总数的约27.7％，已不再具有代表性。总之，本研究筛选并选择了16种ISSR多态性引物，结合基于聚类分析的四个连续的最小距离连续分步采样事件，筛选出19个样品，其比例达到整个核心种质的40.4％。核心种质的高Ne，He和I表明，该策略产生的结果可用于进一步改善仁杏种质。

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