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首页> 外文期刊>International journal of food properties >Characterization of biosynthesized chitosan nanoparticles from Penaeus vannamei for the immobilization of P. vannamei protease: An eco-friendly nanobiocatalyst
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Characterization of biosynthesized chitosan nanoparticles from Penaeus vannamei for the immobilization of P. vannamei protease: An eco-friendly nanobiocatalyst

机译：南美白对虾生物合成壳聚糖纳米粒的表征，用于固定南美白对虾蛋白酶：一种生态友好的纳米生物催化剂

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摘要

Nanomaterials are being applied due to their excellent characteristics, such as the high surface area-to-volume ratio, excellent physicochemical properties, and biological compatibility. Chitosan is an amino polysaccharide and a versatile, second-most abundant natural polymer. In this presented work, chitosan was purified from Penaeus vannamei from the Persian Gulf and the homogeneity level was used a combination of three procedures: deproteinization, demineralization, and deacetylation. Chitosan nanoparticles were synthesized by the ionization gelation technique. P. vannamei protease immobilized on the surface of the positively charged chitosan nanoparticles through ionic or electrostatic interaction. The immobilized enzyme exhibited better tolerance to variations in the medium pH and temperature, enhanced pH and temperature stability, improved storage stability, and good reusability compared with the free enzyme. The Michaelis–Menten kinetic constant (K_(m)), the maximum reaction velocity (V_(max)), and the catalytic efficiency (k_(cat)) for native P. vannamei protease were 2.5 μM, 87 μM. min~(?1), and 132 min~(?1), respectively, whereas for immobilized enzyme, K_(m), V_(max), and k_(cat) values were 2.7 μM, 83 μM. min~(?1), and 128 min~(?1), respectively. The effect of both soluble and immobilized P. vannamei protease on the clarification of orange juice was also analysed.
机译：纳米材料由于其优异的特性而被应用，例如高表面积体积比，出色的理化特性和生物相容性。壳聚糖是一种氨基多糖，是一种多功能的，第二富裕的天然聚合物。在这项提出的工作中，壳聚糖是从波斯湾的凡纳哥对虾中纯化得到的，均一性水平是三个步骤的组合：脱蛋白，脱矿质和脱乙酰化。通过电离凝胶技术合成了壳聚糖纳米颗粒。 P凡纳滨蛋白酶通过离子或静电相互作用固定在带正电荷的壳聚糖纳米颗粒表面。与游离酶相比，固定化酶对培养基pH和温度的变化表现出更好的耐受性，增强了pH和温度稳定性，改善了储存稳定性，并具有良好的可重复使用性。天然P的Michaelis–Menten动力学常数（K_（m）），最大反应速度（V_（max））和催化效率（k_（cat））。凡纳滨蛋白酶分别为2.5μM，87μM。 min _（？1）和132 min〜（？1），而固定化酶的K_（m），V_（max）和k_（cat）值分别为2.7μM，83μM。 min〜（？1）和128 min〜（？1）。可溶性和固定化P的影响。还对南美白对橙汁澄清的蛋白酶进行了分析。

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