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Contaminated operating theatre foot wears: a potential source of healthcare associated infections in a northern Nigerian hospital

机译:受污染的手术室脚部磨损:尼日利亚北部一家医院中与医疗保健相关的潜在感染源

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Operating theatre foot wears (OTFWs) contaminated with potential pathogens are increasingly recognized as possible sources of healthcare associated infections (HCAIs). This study was undertaken to determine the rate of microbial contamination of OTFWs with potential nosocomial pathogens. Sterile cotton wool – tipped swabs momentarily immersed in physiological saline were used to collect samples from parts(i.e. surface and soles) of the OTFWs regularly worn by the operating theater personnel at Murtala Mohammed Specialist Hospital (MMSH), Kano between January and June, 2010. These were cultured by standard procedures and examined for bacterial and fungal growth. Presence of human haemoglobin on non visible blood stained foot wears was confirmed with rapid chromatological immunoassay. Bacterial and fungal isolates were identified by standard microbiological methods Out of a total of 136 pieces of foot wears examined, 56(41.2%) were found with no visible blood stain while blood stain was detected on 80(58.8%). However, greater number 92(68.0%) of foot wears were found to carry blood stain when chemical analysis was adapted. Streptococcus spp. and Staphylococcus epidermidis were most frequently isolated. The findings from this study could be used to adapt appropriate preventive measures in the theater to limit transmission of potential pathogens from HCAIs.
机译:被潜在病原体污染的手术室手术鞋(OTFW)日益被认为是医疗保健相关感染(HCAI)的可能来源。进行这项研究来确定OTFW受到潜在医院病原体的微生物污染率。在2010年1月至2010年6月期间,使用加棉的无菌棉签(暂时浸入生理盐水中)从手术室人员定期穿着的OTFW的部分(即表面和鞋底)中收集样品,这些手术是由手术室人员在卡诺的Murtala Mohammed专科医院(MMSH)进行的。通过标准程序培养它们,并检查细菌和真菌的生长。通过快速色谱免疫测定法可确认人血红蛋白存在于不可见的血染脚上。通过标准的微生物学方法鉴定出细菌和真菌分离物,共检查了136件脚部磨损,其中56件(41.2%)没有可见的血迹,而80件(58.8%)被发现。但是,当对化学分析进行调整时,发现更多的92(68.0%)的脚磨损会留下血迹。链球菌表皮葡萄球菌和表皮葡萄球菌最常分离。这项研究的结果可用于调整战区适当的预防措施,以限制HCAI潜在病原体的传播。

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