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Anaerobic and Aerobic Growth of Bacillus Licheniformis in Gasoline Contaminated Soil of Federal Institute of Industrial Research Oshodi (Fiiro), Lagos, Nigeria

机译:尼日利亚拉各斯联邦工业研究中心Oshodi(Fiiro)的汽油污染土壤中地衣芽孢杆菌的厌氧和有氧生长

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Studies on the anaerobic and aerobic growth of Bacillus licheniformis bacteria show that the same growth factor suffice for both aerobic and anaerobic growth, thus supporting [13, 22] formulation of photosynthesis and carbon assimilation in these forms, and his thesis that the previously observed need for peptone or yeast extract could be attributed to their content of essential growth factors. A bottle technique for anaerobic cultures and a flask technique for aerobic cultures are described in detail. Photosynthesis in Bacillus licheniformis bacteria is best observed when they are grown anaerobic. The experiments described here indicate that although the culture media had to be modified to conform to certain special requirement imposed by anaerobic growth, the required growth factors remained the same. The Bacillus licheniformis bacteria display the same growth pattern in both aerobic and anaerobic condition in ethylene substrate but there was slight change of its growth pattern in both aerobic and anaerobic condition for xylene and benzene substrate which are aromatic compounds. This study shows higher growth of Bacillus licheniformis bacteria in aerobic condition than anaerobic condition. The Bacillus licheniformis bacteria had the highest bacterial population of 1.66×10~7 CFU/g in ethylene substrate for week four in this experiment. The findings suggest that Bacillus licheniformis bacteria is most adapted to conditions present in soils contaminated with gasoline and hence can be exploited in bioremediation activities [18].
机译:对地衣芽孢杆菌细菌的厌氧和好氧生长的研究表明,有氧和厌氧生长都需要相同的生长因子,因此支持这些形式的光合作用和碳同化[13,22],并且他的论文提出了以前观察到的需要蛋白p或酵母提取物的含量可归因于其必需生长因子的含量。详细描述了用于厌氧培养的瓶技术和用于有氧培养的烧瓶技术。地衣芽孢杆菌厌氧生长时最好观察到光合作用。此处描述的实验表明,尽管必须对培养基进行修改以符合厌氧生长带来的某些特殊要求,但所需的生长因子仍保持不变。地衣芽孢杆菌细菌在好氧和厌氧条件下在乙烯基质中均表现出相同的生长方式,但是对于二甲苯和苯基质(芳香化合物),好氧和厌氧条件下地衣芽孢杆菌的生长方式均发生微小变化。这项研究显示在有氧条件下地衣芽孢杆菌细菌比无氧条件下细菌的生长更高。在本实验的第四周中,地衣芽孢杆菌在乙烯底物中的细菌种群最高,为1.66×10〜7 CFU / g。这些发现表明地衣芽孢杆菌细菌最适合存在于被汽油污染的土壤中的条件,因此可以用于生物修复活动[18]。

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