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首页> 外文期刊>International Journal of Molecular Epidemiology and Genetics >Biomarkers measured in buccal and blood leukocyte DNA as proxies for colon tissue global methylation
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Biomarkers measured in buccal and blood leukocyte DNA as proxies for colon tissue global methylation

机译：在颊和血液白细胞DNA中测量的生物标志物作为结肠组织整体甲基化的代理

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摘要

There is increasing interest in clarifying the role of global DNA methylation levels in colorectal cancer (CRC) etiology. Most commonly, in epidemiologic studies, methylation is measured in DNA derived from blood leukocytes as a proxy measure of methylation changes in colon tissue. However, little is known about the correlations between global methylation levels in DNA derived from colon tissue and more accessible tissues such as blood or buccal cells. This cross-sectional study utilized DNA samples from a screening colonoscopy population to determine to what extent LINE-1 methylation levels (as a proxy for genome-wide methylation) in non-target tissue (e.g., blood, buccal cells) reflected methylation patterns of colon mucosal tissue directly at risk of developing CRC. The strongest Pearson correlation was observed between LINE-1 methylation levels in buccal and blood leukocyte DNA (emr/em = 0.50; N = 67), with weaker correlations for comparisons between blood and colon tissue (emr/em = 0.36; N = 280), and buccal and colon tissue (emr/em = 0.27; N = 72). These findings of weak/moderate correlations have important implications for interpreting and planning future investigations of epigenetic markers and CRC risk.
机译：人们越来越关注阐明全球DNA甲基化水平在大肠癌（CRC）病因中的作用。最常见的是，在流行病学研究中，在血液白细胞衍生的DNA中测量甲基化，作为结肠组织甲基化变化的替代指标。然而，人们对结肠组织和更容易接触的组织（例如血液或颊细胞）中的DNA的整体甲基化水平之间的相关性了解甚少。这项横断面研究利用了来自结肠镜检查人群的DNA样品，以确定非靶组织（例如血液，颊细胞）中LINE-1甲基化水平（作为全基因组甲基化的替代指标）反映了结肠粘膜组织直接处于发生CRC的风险中。在颊和血液白细胞DNA的LINE-1甲基化水平之间观察到最强的Pearson相关性（ r = 0.50; N = 67），而在血液和结肠组织之间进行比较的相关性较弱（ r = 0.36; N = 280）以及颊和结肠组织（ r = 0.27; N = 72）。这些弱/中度相关性的发现对于解释和计划表观遗传标记和CRC风险的未来研究具有重要意义。

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