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首页> 外文期刊>International journal of molecular imaging >FDG-PET Quantification of Lung Inflammation with Image-Derived Blood Input Function in Mice
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FDG-PET Quantification of Lung Inflammation with Image-Derived Blood Input Function in Mice

机译：FDG-PET定量化小鼠图像来源的血液输入功能对肺部炎症的影响

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Dynamic FDG-PET imaging was used to study inflammation in lungs of mice following administration of a virulent strain ofKlebsiella (K.) pneumoniae. Net whole-lung FDG influx constant (Ki) was determined in a compartment model using an image-derived blood input function.Methods.K. pneumoniae(~3 x 105CFU) was intratracheally administered to six mice with 6 other mice serving as controls. Dynamic FDG-PET and X-Ray CT scans were acquired 24 hr afterK. pneumoniaeadministration. The experimental lung time activity curves were fitted to a 3-compartment FDG model to obtainKi. Following imaging, lungs were excised and immunohistochemistry analysis was done to assess the relative presence of neutrophils and macrophages.Results. MeanKifor control andK. pneumoniaeinfected mice were (5.1±1.2)×10-3versus (11.4±2.0)×10-3 min−1, respectively, revealing a 2.24 fold significant increase (P=0.0003) in the rate of FDG uptake in the infected lung. Immunohistochemistry revealed that cellular lung infiltrate was almost exclusively neutrophils. ParametricKimaps by Patlak analysis revealed heterogeneous inflammatory foci within infected lungs.Conclusion. The kinetics of FDG uptake in the lungs of mice can be noninvasively quantified by PET with a 3-compartment model approach based on an image-derived input function.
机译：动态FDG-PET成像用于研究强毒性肺炎克雷伯菌（K.）菌株的小鼠肺部炎症。使用图像来源的血液输入函数在隔室模型中确定全肺FDG净流入常数（Ki）。气管内给予六只小鼠肺炎（〜3 x 105CFU），另六只小鼠作为对照。术后24小时进行动态FDG-PET和X射线CT扫描。肺炎的管理。将实验肺时间活性曲线拟合至三室FDG模型以获得Ki。成像后，切除肺并进行免疫组织化学分析以评估中性粒细胞和巨噬细胞的相对存在。 MeanKifor控制和K。感染肺炎的小鼠分别为（5.1±1.2）×10-3比（11.4±2.0）×10-3 min-1，显示受感染肺中FDG摄取速率的显着增加（P = 0.0003）2.24倍。免疫组织化学显示，细胞肺浸润几乎仅是嗜中性粒细胞。 Patlak分析得出的ParametricKimaps显示受感染的肺内存在异质性炎症灶。可以使用基于图像输入功能的3室模型方法，通过PET无创地对小鼠肺中FDG摄取的动力学进行定量。

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