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首页> 外文期刊>International journal of molecular imaging >In VitroandEx VivoEvaluation of Smart Infra-Red Fluorescent Caspase-3 Probes for Molecular Imaging of Cardiovascular Apoptosis
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In VitroandEx VivoEvaluation of Smart Infra-Red Fluorescent Caspase-3 Probes for Molecular Imaging of Cardiovascular Apoptosis

机译：智能红外荧光Caspase-3探针的VitroandEx体内评估，用于心血管细胞凋亡的分子成像

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Purpose. The aim of this paper is to develop new optical bioprobes for the imaging of apoptosis.Procedure. We developed quenched near-infrared probes which become fluorescent upon cleavage by caspase-3, the key regulatory enzyme of apoptosis.Results. Probes were shown to be selectively cleaved by recombinant caspase-3. Apoptosis of cultured endothelial cells was associated with an increased fluorescent signal for the cleaved probes, which colocalized with caspase-3 and was reduced by the addition of a caspase-3 inhibitor. Flow cytometry demonstrated a similar profile between the cleaved probes and annexin V.Ex vivoexperiments showed that sections of hearts obtained from mice treated with the proapoptotic drug doxorubicin displayed an increase in the fluorescent signal for the cleaved probes, which was reduced by a caspase-3 inhibitor.Conclusion. We demonstrated the capacity of these novel probes to detect apoptosis by optical imagingin vitroandex vivo.
机译：目的。本文的目的是开发新的光学生物探针用于细胞凋亡的成像。我们开发了淬灭的近红外探针，该探针在被凋亡的关键调节酶caspase-3裂解后会发荧光。探针被重组caspase-3选择性切割。培养的内皮细胞的凋亡与被切割探针的荧光信号增加有关，该信号与caspase-3共定位，并通过添加caspase-3抑制剂而减少。流式细胞仪显示裂解的探针和膜联蛋白V之间具有相似的特征。离体实验显示，从用促凋亡药物阿霉素处理的小鼠获得的心脏切片显示裂解的探针的荧光信号增加，而caspase-3降低了荧光信号抑制剂。结论。我们证明了这些新型探针通过体外和体内光学成像检测凋亡的能力。

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