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首页> 外文期刊>International journal of molecular imaging >Concurrent Dual Contrast for Cellular Magnetic Resonance Imaging Using Gadolinium Oxide and Iron Oxide Nanoparticles
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Concurrent Dual Contrast for Cellular Magnetic Resonance Imaging Using Gadolinium Oxide and Iron Oxide Nanoparticles

机译：氧化Dual和氧化铁纳米粒子的细胞核磁共振成像的并发双重对比度。

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Rationale and Objectives. Concurrent visualization of differential targets in cellular and molecular imaging is valuable for resolving processes spatially and temporally, as in monitoring different cell subtypes. The purpose of this study was to demonstrate concurrent, dual (positive and negative) contrast visualization on magnetic resonance imaging (MRI) of two colocalized cell populations labeled with Gadolinium “Gd” oxide and iron “Fe” oxide nanoparticles.Materials and Methods. Human aortic endothelial cells (EC) and smooth muscle cells (SMC) were labeled with various concentrations of Gd oxide and Fe oxide, respectively. MRI on single- or mixed-cell samples was performed at 7 tesla. Proper cell phenotype expressions, cell uptake of contrast agents, and the effect of labeling on cell viability and proliferation were also determined.Results. Both contrast agents were efficiently taken up by cells, with viability and proliferation largely unaffected. On MRI, the positive contrast associated with Gd oxide-labeled EC and negative contrast associated with Fe oxide-labeled SMC discriminated the presence of each cell type, whether it existed alone or colocalized in a mixed-cell sample.Conclusion. It is feasible to use Gd oxide and Fe oxide for dual contrast and concurrent discrimination of two colocalized cell populations on MRI at 7 tesla.
机译：基本原理和目标。同时在细胞和分子成像中可视化不同目标的可视化对于在空间和时间上解决过程非常有价值，例如在监视不同的细胞亚型中。这项研究的目的是证明在同时成像的两个共定位细胞群的磁共振成像（MRI）上同时进行双（正和负）对比可视化，这些细胞群分别标记了氧化“和氧化铁纳米粒子。材料和方法。用不同浓度的氧化（和氧化铁分别标记人主动脉内皮细胞（EC）和平滑肌细胞（SMC）。在7特斯拉对单细胞或混合细胞样品进行MRI。还确定了正确的细胞表型表达，造影剂的细胞摄取以及标记对细胞活力和增殖的影响。两种造影剂均能有效地被细胞吸收，并且活力和增殖在很大程度上不受影响。在MRI上，与Gd氧化物标记的EC相关的正对比和与Fe氧化物标记的SMC相关的负对比可区分每种细胞类型的存在，无论是单独存在还是在混合细胞样本中共存。在7特斯拉的MRI上，使用Gd氧化物和Fe氧化物进行双重对比和同时鉴别两个共定位的细胞群是可行的。

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