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首页> 外文期刊>International Journal of Molecular Epidemiology and Genetics >Multi-Locus Sequence Typing (MLST) and Repetitive Extragenic Palindromic Polymerase Chain Reaction (REP-PCR), characterization of shigella spp. over two decades in Tianjin China
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Multi-Locus Sequence Typing (MLST) and Repetitive Extragenic Palindromic Polymerase Chain Reaction (REP-PCR), characterization of shigella spp. over two decades in Tianjin China

机译：多基因座序列分型（MLST）和重复性外源性回文聚合酶链反应（REP-PCR），对志贺氏菌属进行了鉴定。在中国天津超过二十年

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To understand the change of the dominant serogroup of Shigella spp., their antimicrobial resistance over more than two decades in Tianjin, their phylogenetic similarity and to determine their evolutionary biology by using REP-PCR and MLST in order to study their epidemiological character. Multi-locus Sequence Typing was performed to determine their lineage and phylogenetic similarity. REP-PCR typing was used to study the homology of their genomic DNA. The isolated rate of group D Shigella in 2009 and 2010 had obviously increased. Antimicrobial susceptibility test results showed that the resistant rates of the 1981-1983 Shigella flexneri to tetracycline, streptomycin and chloramphenicol varied from 76.47 to 100%, they were all sensitive to other antibiotics. During 2009-2010, the resistance rates of the isolated Shigella flexneri to gentamicin, amikacin, third and fourth Generation Cephalosporins and quinolones had increased. MLST results produced five sequence types and two sequence type complexes. REP-PCR showed DNA band similarities between the 1981-1983 and 2009-2010 strains. The dominant serogroup of Shigella in Tianjin has changed from Shigella flexneri to Shigella sonnei. Increased drug resistance of Shigella flexneri is higher than Shigella sonnei because a great variety of antibiotics has been used. The MLST results showed that the 1981-1983 strains had the same sequence type with some of the 2009-2010 strains. Combination of MLST and REP-PCR produced better discriminatory power than using either method alone.
机译：为了了解志贺氏菌主要血清群的变化，天津地区超过二十年的抗药性，系统发育相似性以及通过REP-PCR和MLST来确定其进化生物学，以研究其流行病学特征。进行多基因座序列分型以确定其谱系和系统发育相似性。 REP-PCR分型用于研究其基因组DNA的同源性。 D群志贺氏菌在2009年和2010年的隔离率明显提高。抗菌药敏试验结果表明，1981-1983年弗氏志贺氏菌对四环素，链霉素和氯霉素的耐药率从76.47到100％不等，它们对其他抗生素均敏感。在2009-2010年期间，分离的弗氏志贺氏菌对庆大霉素，丁胺卡那霉素，第三和第四代头孢菌素和喹诺酮类药物的耐药率有所增加。 MLST结果产生了五个序列类型和两个序列类型复合体。 REP-PCR显示1981-1983年和2009-2010年菌株之间的DNA条带相似性。天津志贺氏菌的主要血清群已从弗氏志贺氏菌变为索氏志贺氏菌。弗氏志贺氏菌的耐药性要高于索氏志贺氏菌，因为已经使用了多种抗生素。 MLST结果表明1981-1983菌株与某些2009-2010菌株具有相同的序列类型。 MLST和REP-PCR的组合比单独使用任何一种方法产生更好的区分能力。

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